Estimation of protein adsorption on dialysis membrane by means of TOF-SIMS imaging
Estimation of protein adsorption on dialysis membrane by means of TOF-SIMS imaging
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利用 TOF-SIMS 成像估算透析膜上的蛋白质吸附
DOI：
10.1016/j.memsci.2004.02.010
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发表时间：
2004
影响因子：
9.5
通讯作者：
M. Kudo
中科院分区：
文献类型：
--
作者：
S. Aoyagi;M. Hayama;U. Hasegawa;K. Sakai;M. Tozu;T. Hoshi;M. Kudo
通过飞行时间二次离子质谱（TOF-SIMS）研究了透析膜上的蛋白质吸附，该方法有望对绝缘样品（例如中空纤维透析膜）进行化学成像。本研究测试了三种具有不同孔径和结构的市售中空纤维透析膜。使用牛血清白蛋白（BSA）溶液来吸附样品上的蛋白质。对天然膜和用 BSA 处理的膜的 TOF-SIMS 图像和光谱进行比较，以识别与 BSA 和膜相关的二次离子。互信息是信息论的应用之一，用于选择与 BSA 相关的碎片离子。 TOF-SIMS 图像显示了透析膜上吸附的 BSA 分布，表明 BSA 渗透性以及膜与 BSA 之间的相互作用绝对取决于孔径、结构和材料。
Protein adsorption on dialysis membrane was studied by time-of-flight secondary ion mass spectrometry (TOF-SIMS), which is expected to be capable of chemical imaging of insulated samples, such as hollow-fiber dialysis membranes. Three commercially available hollow-fiber dialysis membranes having different pore sizes and structures, were tested in the present study. Bovine serum albumin (BSA) solutions were used to adsorb the protein on the samples. TOF-SIMS images and spectra of native membranes and membranes treated with BSA were compared in order to identify secondary ions related to BSA and membranes. Mutual information, one of applications of information theory, was employed to select fragment ions related to BSA. TOF-SIMS images show distribution of adsorbed BSA on the dialysis membranes and indicate that BSA permeability and interaction between the membranes and BSA definitely depend on pore size, structure and material.
通过多变量模式识别解释吸附蛋白膜的静态飞行时间二次离子质谱。
影响因子：
7.4
作者：
Wagner,MS;Tyler,BJ;Castner,DavidG
通讯作者：
Castner,DavidG