Ni-NTA Affinity Chromatography to Characterize Protein-Protein Interactions During Fe-S Cluster Biogenesis.
Ni-NTA Affinity Chromatography to Characterize Protein-Protein Interactions During Fe-S Cluster Biogenesis.
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DOI：
10.1007/978-1-0716-1605-5_7
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Outten FW
中科院分区：
文献类型：
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作者：
Carter TD;Outten FW
亲和层析可以重新调整用途，以提供有关目标蛋白可能结合的特定伴侣蛋白以及该伴侣蛋白与目标蛋白的相对结合亲和力的有用信息。在这里，我们提供了 Ni-NTA 亲和层析测定的方案，可用于揭示铁硫 (Fe-S) 簇生物发生反应期间蛋白质-蛋白质相互作用性质的深入信息。
Affinity chromatography can be repurposed to provide useful information about the specific partner protein (s) to which a protein of interest may bind as well as the relative binding affinity of that partner protein for the protein of interest. Here, we provide a protocol for an Ni-NTA affinity chromatography assay that may be utilized to uncover insightful information about the nature of protein–protein interactions during iron-sulfur (Fe-S) cluster biogenesis reactions.