Establishment and Characterization of MACL-1 and MGSO-3 Cell Lines Derived From Human Primary Breast Cancer
Establishment and Characterization of MACL-1 and MGSO-3 Cell Lines Derived From Human Primary Breast Cancer
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DOI：
10.3727/096504009789735404
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发表时间：
2009-01-01
影响因子：
3.1
通讯作者：
Goes, A. M.
中科院分区：
文献类型：
--
作者：
Correa, C. R.;Bertollo, C. M.;Goes, A. M.
乳腺癌是世界范围内的主要健康负担。它是世界上1 000万癌症病例中的100多万例的原因。乳腺癌检测和治疗的进展有助于提高生存率，尽管死亡率仍然很高。尽管有这些进步，更有效的诊断方法和有效的治疗是必要的。乳腺癌细胞系的建立是了解这种疾病所涉及的生物学过程以及识别潜在治疗靶点的重要工具。本研究采用差速离心法从人原发性乳腺癌细胞中建立了MACL-1和MGSO-3两株细胞系，并进行了传代培养。细胞系的表征包括形态学分析、倍增时间测定、端粒酶表达、肿瘤抗原表达、软琼脂集落形成和裸鼠异种移植。形态学检查显示为典型的上皮细胞形态，PCR分析显示两种细胞系均为端粒酶阳性。此外，MACL-1和MGSO-3能够在软琼脂培养物中生长，这表明其具有转移潜力，并且在裸鼠中均表现出阳性致瘤潜力。这些实验模型为乳腺癌病理生物学的研究开辟了新的视角。
Breast cancer is a major health burden worldwide. It is responsible for over 1 million of 10 million cases of cancer in the world. Advances in breast cancer detection and treatment have contributed to improve the rate of survival, although mortality rates remains significantly high. Despite all these advances, more efficient diagnostic methods and effective treatments are necessary. The establishment of breast cancer cell lines is an important tool to understand biological processes involved in this disease, as well as the identification of potential therapeutic targets. In the present work, two cell lines, MACL-1 and MGSO-3, were established from human primary breast cancer based on differential centrifugation, followed by growth in culture for over 70 passages. Characterization of the cell lines included morphology analysis, determination of doubling time, telomerase expression, tumor antigen expression, colony formation in soft agar, and xenograft implantation into nude mice. Morphological examination demonstrated a typical epithelial morphology and PCR analyses showed that both cell lines were telomerase positives. Moreover, MACL-1 and MGSO-3 were capable of growing in soft agar culture, which suggests its metastatic potential, and both demonstrated a positive tumorigenic potential in nude mice. These experimental models open new perspectives on the investigation of breast cancer pathobiology.