Strategies to minimize background autofluorescence in live mice during noninvasive fluorescence optical imaging
Strategies to minimize background autofluorescence in live mice during noninvasive fluorescence optical imaging
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DOI：
10.1038/laban0907-40
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发表时间：
2007-09-01
期刊：
影响因子：
6.9
通讯作者：
Klimash, June
中科院分区：
文献类型：
--
作者：
Bhaumik, Srabani;DePuy, Jeannette;Klimash, June
随着小动物荧光成像越来越多地被广泛的用户使用，许多实验室动物研究人员才刚刚开始面临其挑战。荧光成像的一个挫折是在动物组织和摄入的食物中产生的背景自体荧光。作者将这一问题纳入焦点，并展示了如何通过选择适当的激发波长和小鼠饮食来减少裸鼠的自体荧光。
As small-animaL fluorescence imaging becomes increasingly accessible to a broad spectrum of users, many Lab animal researchers are just beginning to be exposed to its challenges. One setback to fluorescence imaging is background autofLuorescence generated in animal tissue and in ingested food. The authors bring this issue into focus, and show how autoftuorescence can be reduced in nude mice through selection of appropriate excitation wavelength and mouse diet.