Expression of platelet-derived growth factor and its receptor in livers of patients with chronic liver disease
Expression of platelet-derived growth factor and its receptor in livers of patients with chronic liver disease
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DOI：
10.1007/bf02934089
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发表时间：
1997-08-01
影响因子：
6.3
通讯作者：
Sakurai, M
中科院分区：
文献类型：
--
作者：
Ikura, Y;Morimoto, H;Sakurai, M
为了发现血小板衍生生长因子是否有助于慢性肝病中的肝纤维化，我们研究了慢性肝炎或肝硬化患者肝脏中该细胞因子及其β受体的B链表达。本研究纳入了17例患者。5例无肝病者尸检肝组织标本作为对照，用单克隆抗体免疫组化技术鉴定肽的位置。通过原位杂交评估B链的mRNA表达。对B链染色并表达其mRNA的细胞被鉴定为巨噬细胞。在对照组织中，仅少数细胞被染色。在患者的标本中，大多数染色细胞位于汇管区，其数量随着组织学肝损伤而增加。在小叶内区域，在局灶性坏死区域观察到染色细胞。门静脉间充质细胞和窦周细胞表达P受体。这些细胞密集在门静脉周围区域，在那里看到许多肌纤维母细胞样细胞。这些发现表明血小板衍生生长因子的B链主要由参与炎症反应的巨噬细胞释放。这种细胞因子可能作用于成肌纤维细胞样间充质细胞，并可能与慢性肝病的肝纤维化有关。
To find if platelet-derived growth factor contributes to liver fibrosis in chronic liver disease, we studied the expression of the B-chain of this cytokine and its beta-receptor in livers of patients with chronic hepatitis or cirrhosis. Seventeen patients were included in this study. Five specimens of liver tissue obtained during autopsy from subjects without liver disease were used as controls, The location of the peptides was identified by an immunohistochemical technique with monoclonal antibodies. Expression of mRNA for the B-chain was assessed by in situ hybridization. Cells stained for the B-chain and expressing its mRNA were identified as macrophages. In control tissues, only a few cells were stained. In the patients' specimens, most stained cells were in portal areas and their number increased with histologic liver damage. In intralobular areas, the stained cells were seen in regions of focal necrosis. Portal mesenchymal and perisinusoidal cells expressed Preceptor. These cells were dense in periportal areas, where many myofibroblast-like cells were seen. These findings suggest that the B-chain of platelet-derived growth factor is released mainly by macrophages involved in inflammatory reactions. This cytokine probably acts on myofibroblast-like mesenchymal cells, and may be implicated in liver fibrosis in chronic liver disease.