Differences in respiration rates and abrasion losses may muddle attribution of breakdown to macroinvertebrates versus microbes in litterbag experiments
Differences in respiration rates and abrasion losses may muddle attribution of breakdown to macroinvertebrates versus microbes in litterbag experiments
复制标题
呼吸速率和磨损损失的差异可能会混淆垃圾袋实验中大型无脊椎动物与微生物的分解归因
DOI：
10.1002/rra.4055
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发表时间：
2022
影响因子：
2.2
通讯作者：
Wenger, Seth J.
中科院分区：
文献类型：
--
作者：
Tomczyk, Nathan J.;Rosemond, Amy D.;Bumpers, Phillip M.;Cummins, Carolyn S.;Yang, Carol;Wenger, Seth J.
树叶破碎是森林源溪流的一个重要过程。量化大型无脊椎动物和微生物群落在凋落叶分解中的作用的一种常用方法是使用配对的网袋，允许或禁止大型无脊椎动物接近树叶。我们检查了配对垃圾袋方法的常见假设，以测试(1)网目大小是否会改变微生物呼吸；(2)粗网和细网垃圾袋之间的磨料流（例如，来自水和沉积物）的影响是否不同。我们测量了在粗网和细网垃圾袋中培养的红杜鹃叶片的微生物呼吸速率。我们还测量了磨损率，使用加气混凝土块成对的粗网袋和细网袋，在十种水流中跨越流量梯度。我们发现，在细网袋中处理的红毛叶上的微生物呼吸速率比在配对的粗网袋中的呼吸速率高65%，但在粗网袋和细网袋中处理的最大杜鹃花的呼吸速率相似。粗网袋的磨损平均比细网袋高56%，在流量较大的河流中，这种影响更大。这些结果表明，在将粗网袋和细网袋之间的叶片分解差异归因于大型无脊椎动物时，需要更加谨慎。由于网格尺寸对叶片微生物呼吸和磨损的影响方向相反，因此占主导地位并产生偏差的影响可能取决于环境背景和实验设计。
Leaf breakdown is an important process in forested headwater streams. A common method used to quantify the role of macroinvertebrate and microbial communities in leaf litter breakdown involves using paired mesh bags that either allow or exclude macroinvertebrate access to leaves. We examined common assumptions of the paired litterbag method to test (1) whether mesh size alters microbial respiration and (2) whether the effects of abrasive flows (e.g., from water and sediment) differ between coarse‐ and fine‐mesh litterbags. We measured rates of microbial respiration onAcer rubrumandRhododendron maximumleaves incubated in coarse‐ and fine‐mesh litterbags. We also measured rates of abrasion using aerated concrete blocks in pairs of coarse‐ and fine‐mesh bags in ten streams across a gradient of discharge. We found that rates of microbial respiration onAcer rubrumleaves conditioned in fine‐mesh bags were 65% greater than the rates of respiration in paired coarse‐mesh bags, but respiration rates onRhododendron maximumwere similar in coarse‐ and fine‐mesh bags. Abrasion was, on average, 56% greater in coarse‐mesh than paired fine‐mesh bags, and these effects were greater in streams with higher discharge. These results suggest that more caution is required when attributing the difference in leaf breakdown between coarse‐ and fine‐mesh bags to macroinvertebrates. Because the effect of mesh size on microbial respiration ofAcerleaves and abrasion are opposite in direction, the effect that dominates and creates bias likely depends on both environmental context and experimental design.
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