Binding of Hg2+ with Phytochelatins: Study by Differential Pulse Voltammetry on Rotating Au-Disk Electrode, Electrospray Ionization Mass-Spectrometry, and Isothermal Titration Calorimetry
Binding of Hg2+ with Phytochelatins: Study by Differential Pulse Voltammetry on Rotating Au-Disk Electrode, Electrospray Ionization Mass-Spectrometry, and Isothermal Titration Calorimetry
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DOI：
10.1021/es901325f
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发表时间：
2009-09-15
影响因子：
11.4
通讯作者：
Esteban, Miquel
中科院分区：
文献类型：
--
作者：
Chekmeneva, Elena;Manuel Diaz-Cruz, Jose;Esteban, Miquel
用旋转金盘电极上的差示脉冲伏安法、电喷雾电离质谱仪(ESI-MS)和恒温滴定量热法(ITC)研究了人工合成的植物螯合素((γ-Glu-Cys)(N)-Gly，PCn，n=2，3，4)与Hg2+的结合。ESI-MS实验提供了在不同PCN/Hg2+比例下形成的络合物的准确化学计量比。伏安法通过使用多变量曲线分辨率交替使用从滴定获得的数据矩阵的最小二乘与金属或配体的细微增量来提供关于络合过程的更详细的信息。用伏安法研究了Hg2+-GSH-PC2体系，以估算Hg2+在两种相关配体存在下的行为。通过ITC，在Hg2+-PCN体系中使用治疗性螯合剂2，3-二硫代丙磺酸钠(DMPS)，实现了对Hg2+-PCN络合物稳定性的进一步评估。考察了各种Hg2+-PCN络合物的稳定性以及DMPS取代PCN的能力。
The binding of Hg2+ with synthetic phytochelatins ((gamma-Glu-Cys)(n)-Gly, PCn, n = 2, 3, 4) was investigated by a recently proposed electroanalytical method, using differential pulse voltammetry on the rotating Au-disk electrode, Electrospray ionization mass-spectrometry (ESI-MS) and isothermal titration calorimetry (ITC). ESI-MS experiments provided the exact stoichiometries of the complexes formed at different PCn/Hg2+ ratios. Voltammetry provided more detailed information on the complexation processes through the use of multivariate curve resolution by alternating least squares of the data matrix obtained from titrations with fine increments of metal or ligand. The system Hg2+-GSH-PC2 Was investigated by voltammetry in order to obtain an estimation of the Hg2+ behavior in the presence of two related ligands. The additional assessment of the stability of Hg2+-PCn complexes was achieved through ITC by using the therapeutic chelator sodium 2,3-dimercaptopropanesulfate (DMPS) over Hg2+-PCn systems. The stability of various Hg2+-PCn complexes and the ability of DMPS to replace PCn from these complexes were examined.