Inheritance of garden rose architecture and its association with flowering behaviour
Inheritance of garden rose architecture and its association with flowering behaviour
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DOI：
10.1007/s11295-015-0844-3
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发表时间：
2015-04-01
影响因子：
2.4
通讯作者：
Foucher, Fabrice
中科院分区：
文献类型：
--
作者：
Kawamura, Koji;Hibrand-Saint Oyant, Laurence;Foucher, Fabrice
了解植物结构的遗传基础是有限的木本植物，由于评估其复杂的结构遗传的挑战。我们的目的是评估植物的形状和身高在一个花园玫瑰种群的遗传变异，分析遗传的植物结构及其与开花行为的联系，并确定控制花园玫瑰结构的数量性状基因座（QTL）。共98个F-1杂种来自两个二倍体玫瑰，仙女（TF）和玫瑰x wichurana（RW），不同的身高和开花行为之间的杂交。TF具有连续开花（CF）和直立的身材。在一次开花（OF）和匍匐在身材。将每个基因型的三个克隆繁殖，并且在田地中栽培总共300株植物。开花行为，植株形态，株高，茎粗和节间长度在2年期间进行了评分。所有的建筑性状都有显著的遗传方差（占各自表型方差的29-61%），广义遗传力估计值为0.76-0.92。大多数CF后代是直立的，而OF后代是匍匐的，这表明植物形态和开花行为之间的联系。QTL分析确定了8个控制建筑性状的主效QTL。在QTL附近鉴定了几个参与赤霉素生物合成和生长素信号传导的候选基因。高遗传力的估计得到的园林玫瑰的架构表明，架构特性是可行的目标，玫瑰育种。然而，植物形态和开花行为的连锁阻止了这些性状的独立选择。所鉴定的候选基因可以为未来的生理学研究提供良好的靶点。
Understanding the genetic basis of plant architecture is limited for woody plants due to the challenges of assessing the inheritance of their complex architecture. We aimed to evaluate the genetic variability of plant form and stature in a garden rose population, analyse the inheritance of plant architecture and its linkage with flowering behaviour and identify the quantitative trait loci (QTLs) controlling garden rose architecture. A total of 98 F-1 hybrids were derived from the cross between two diploid roses, The Fairy (TF) and Rosa x wichurana (RW) that differed in stature and flowering behaviour. The TF exhibits continuous flowering (CF) and has erect stature. The RW is once flowering (OF) and prostrate in stature. Three clones per genotype were multiplied, and a total of 300 plants were cultivated in a field. Flowering behaviour, plant form, plant height, stem diameter and internode length were scored during each of 2 years. All architectural traits had significant genetic variances (29-61% of their respective phenotypic variances), and their broad-sense heritability estimates were 0.76-0.92. The majority of CF progeny was erect, whereas the OF progeny was prostrate, suggesting a linkage between plant form and flowering behaviour. The QTL analysis identified eight major QTLs controlling architectural traits. Several candidate genes involved in gibberellin biosynthesis and auxin signalling were identified in the vicinity of the QTLs. High heritability estimates obtained for garden rose architecture indicated that architectural characteristics are feasible targets of rose breeding. Linkage of plant form and flowering behaviour, however, prevents independent selection of these traits. The candidate genes identified can be good targets for future physiological studies.