Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products
Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products
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DOI：
10.3390/ijms20194802
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Joddar, Binata
中科院分区：
文献类型：
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作者：
Alvarez-Primo, Fabian;Kumar, Shweta Anil;Joddar, Binata
在这项研究中，我们设计，合成和表征高纯度的单壁碳纳米管（SWCNT）-海藻酸钠水凝胶复合材料。在形成具有单壁碳纳米管的基于藻酸盐的水凝胶复合物中重要的参数是它们的不同纯度、它们的颗粒团聚和它们与细胞活力的剂量依赖性相关性，所有这些都对所得复合物的效率和对细胞治疗的有效性有影响。为了促进其均匀分散，防止团聚的单壁碳纳米管，三种不同的表面活性剂-十二烷基硫酸钠（SDS-阴离子），十六烷基三甲基溴化铵（CTAB-阳离子），和普朗尼克F108（非离子）-被利用。在将SWCNT-表面活性剂与藻酸盐混合后，使用二价钙离子交联混合物，并使用拉曼光谱进行表征。流变学分析表明，与纯海藻酸盐凝胶相比，单壁碳纳米管复合凝胶的复数粘度、损耗和储能模量增加。扫描电子显微镜显示存在一个分布均匀的多孔结构，和所有的单壁碳纳米管-凝胶复合材料描绘增强的电导率相对于藻酸盐凝胶。为了表征其生物相容性，在这些SWCNT-凝胶上培养心肌细胞。结果全面地表明，Pluronic F108在防止藻酸盐基质中的单壁碳纳米管团聚方面是最有效的，从而导致稳定的支架形成，而不会对细胞造成任何毒性。
In this study, we designed, synthesized, and characterized ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites. Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite's efficiency and effectiveness towards cell-therapy. To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized. After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy. Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels. Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels. To characterize their biocompatibility, cardiomyocytes were cultured atop these SWCNT-gels. Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.