Secretagogue-induced formation of inositol phosphates in rat exocrine pancreas. Implications for a messenger role for inositol trisphosphate.
Secretagogue-induced formation of inositol phosphates in rat exocrine pancreas. Implications for a messenger role for inositol trisphosphate.
复制标题
促分泌素诱导大鼠外分泌胰腺中肌醇磷酸盐的形成。
DOI：
10.1042/bj2190655
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
PutneyJr,JW
中科院分区：
文献类型：
--
作者：
Rubin,RP;Godfrey,PP;Chapman,DA;PutneyJr,JW
本文研究了用[~ 3 H]肌醇预孵育的大鼠胰腺腺泡对促分泌素反应的肌醇磷酸的形成。卡巴胆碱引起放射性肌醇磷酸，肌醇二磷酸和肌醇三磷酸迅速增加。这种作用可被阿托品阻断，也可被雨蛙素诱导，但不能被离子霉素或佛波醇二丁酸酯阻断。因此，磷脂酶C介导的多磷酸肌醇的分解，从而形成肌醇磷酸，可能是胰腺外分泌刺激分泌耦合途径的早期步骤。三磷酸肌醇可能作为胰腺外分泌的第二信使，将受体激活与内部Ca 2+释放偶联。
The formation of inositol phosphates in response to secretagogues was studied in rat pancreatic acini preincubated with [3H]inositol. Carbachol caused rapid increases in radioactive inositol phosphate, inositol bisphosphate and inositol trisphosphate . This effect was blocked by atropine, and also elicited by caerulein, but not by ionomycin or phorbol dibutyrate. Thus phospholipase C-mediated breakdown of polyphosphoinositides, with the resulting formation of inositol phosphates, may be an early step in the stimulus-secretion coupling pathway in exocrine pancreas. Inositol trisphosphate may function as a second messenger in the exocrine pancreas, coupling receptor activation to internal Ca2+ release.