The oriental armyworm (Mythimna separata) feeding induces systemic defence responses within and between maize leaves
The oriental armyworm (Mythimna separata) feeding induces systemic defence responses within and between maize leaves
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东方粘虫（Mythimna separata）的摄食会诱导玉米叶内和叶间的系统防御反应
DOI：
10.1098/rstb.2018.0307
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发表时间：
2019
影响因子：
6.3
通讯作者：
Wu Jianqiang
中科院分区：
文献类型：
--
作者：
Malook Saif ul;Qi Jinfeng;Hettenhausen Christian;Xu Yuxing;Zhang Cuiping;Zhang Jingxiong;Lu Chengkai;Li Jing;Wang Lei;Wu Jianqiang
玉米（Zea mays）是世界各地种植的主要谷类作物，但受到各种昆虫的威胁。取食鳞翅目昆虫Mythimna separata会触发防御信号，并增加受昆虫损害的玉米叶片中的抗草食动物苯并恶嗪类化合物（BXS）。然而，在玉米中，食草诱导的叶内和叶到叶的系统性信号传导在很大程度上仍未被探索。在这里，我们表明，模拟M。separata植食性和机械损伤引起茉莉酸（JA）、JA-Ile（JA-异亮氨酸缀合物）和Bxs在受损区域和叶内特定系统区域中水平增加。重要的是，在系统叶中检测到Bxs含量增加，并且一致地，该叶表现出对M的防御增加。分离。增加JA/JA-Ile和改变的转录组，包括Bx生物合成基因，检测到系统叶片受伤或模拟植食动物处理后，虽然只有模拟植食动物诱导的Bxs含量增加系统。启动子和共表达分析表明，转录因子bHLH 57和WRKY 34可能调控系统叶中Bx生物合成基因的表达。此外，叶片消融实验表明，系统信号在激发后30 min内迅速离开局部叶片。本研究为玉米对鳞翅目昆虫防御反应的时空调控提供了新的认识。这篇文章是“生物信号揭示了智能害虫管理”主题的一部分。
Maize (Zea mays) is a staple cereal crop cultivated all over the world but that is threatened by various insects. Feeding of the lepidopteran insect Mythimna separata triggers defence signalling and increases anti-herbivore benzoxazinoids (Bxs) in the insect-damaged maize leaves. However, the herbivory-elicited within-leaf and leaf-to-leaf systemic signalling in maize remains largely unexplored. Here, we show that simulated M. separata herbivory and mechanical wounding elicited increased levels of jasmonic acid (JA), JA–Ile (JA–isoleucine conjugate) and Bxs in the damaged areas and in specific systemic regions within a leaf. Importantly, increased contents of Bxs were detected in a systemic leaf, and consistently, this leaf exhibited increased defence against M. separata. Increased JA/JA–Ile and altered transcriptome, including Bx biosynthesis genes, were detected in systemic leaves after wounding or simulated herbivory treatments, although only simulated herbivory induced increase of the contents of Bxs systemically. Promoter and co-expression analysis revealed that transcription factors bHLH57 and WRKY34 may regulate Bx biosynthesis genes in systemic leaves. Moreover, leaf ablation experiment indicated that the systemic signal rapidly exited the local leaves within 30 min after elicitation. This study provides new insight into the temporal and spatial regulation of defence responses of maize against lepidopteran insects. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.