EFFECTS OF LOW-LEVEL X-IRRADIATION ON CAT CEREBELLA AT DIFFERENT POSTNATAL INTERVALS .2. CHANGES IN PURKINJE-CELL MORPHOLOGY
EFFECTS OF LOW-LEVEL X-IRRADIATION ON CAT CEREBELLA AT DIFFERENT POSTNATAL INTERVALS .2. CHANGES IN PURKINJE-CELL MORPHOLOGY
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DOI：
10.1002/cne.901710104
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发表时间：
1977-01-01
影响因子：
2.5
通讯作者：
STROMBERG, MW
中科院分区：
文献类型：
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作者：
ANDERSON, WJ;STROMBERG, MW
用150 R和200 R的分次剂量在出生后的不同时间段照射幼猫的整个头部。实验年龄条件包括辐照新生儿，1-wk，2-wk，3-wk和4-wk的年龄条件，而牺牲的年龄保持不变，在70天。新生儿的分子层厚度减少了47%，1周组减少了40%，2周组减少了17%，3周组减少了19%，4周组减少了9%。高尔基浸渍材料的评价显示，浦肯野细胞的树突状分支一致减少的年龄越早，在辐射开始。在所有的实验条件下，看到减少多刺的小枝。所有的实验年龄条件下显示的现象，树突筑坝软膜表面，其中包括一个过度拥挤的多刺的分支在这个水平。新生儿组和1周组的初生树突在分支发生前生长增加。这在2周组中变得更少，但仍然显著。讨论了这种现象与星状细胞存在或缺乏的相关性。对从其索马胞体中出现2个或更多树突的浦肯野细胞进行分类和单独分析。这些细胞中的初级树突分支通常遵循不同的形态学模式，并且彼此独立。爬行纤维符合浦肯野细胞的异常树突状分支，并在早期放射治疗组的复杂性降低。攀缘纤维对树突状细胞的生长模式没有影响，而是受到浦肯野细胞树突状细胞生长的影响。
The whole-head of infant kittens was irradiated with fractionated doses of 150 R and 200 R at different postnatal intervals. Experimental age conditions consisted of an irradiated newborn, 1-wk, 2-wk, 3-wk and a 4-wk age condition while the age of sacrifice remained constant at 70 days. The molecular layer thickness was reduced by 47% in the newborn, 40% in the 1-wk group, 17% in the 2-wk group, 19% in the 3-wk group and by 9% in the 4-wk group. An evaluation of Golgi impregnated material revealed that the dendritic arborizations of Purkinje cells were consistently reduced the earlier the age at which radiation was begun. A reduction in spiny branchlets was seen in all of the experimental conditions. All experimental age conditions displayed the phenomenon of dendritic damming at the pial surface, which consisted of an excessive crowding of spiny branches at this level. An increased growth of the primary dendrite before branching took place was seen in the newborn and 1-wk group. This became less but was still significant in the 2-wk group. A correlation of this phenomenon with the presence or lack of stellate cells is discussed. Purkinje cells with 2 or more dendrites emerging from their soma were classified and analyzed separately. The primary dendritic branching in these cells often followed separate morphological patterns and appeared independent of each other. Climbing fibers conformed to the abnormal dendritic arborizations of the Purkinje cells, and were reduced in complexity in the early radiation treatment groups. Climbing fibers had no influence upon the dendritic growth pattern, but instead were under the influence of the Purkinje cell dendritic growth.