Characterization of activity-dependent changes in flavoprotein fluorescence in cerebellar slices from juvenile rats.
Characterization of activity-dependent changes in flavoprotein fluorescence in cerebellar slices from juvenile rats.
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DOI：
10.1016/j.neulet.2014.09.052
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Valenzuela CF
中科院分区：
文献类型：
--
作者：
Jotty K;Shuttleworth CW;Valenzuela CF
归因于神经元代谢的黄素蛋白自发荧光信号已被用于评估突触功能。在这里，我们的特点黄素蛋白自发荧光反应的分子层大鼠小脑切片。高频刺激引起瞬时荧光增加（峰值阶段），随后是持续时间较长的荧光减少（谷值阶段）。峰相仅限于分子层，而谷相延伸到浦肯野细胞层和部分颗粒细胞层。通过Na+通道拮抗剂河豚毒素或AMPA受体拮抗剂NBQX和GIKI-53655的组合消除反应，并且还通过黄素蛋白抑制剂（diphenyleneiodonium）减少反应。这些发现与AMPA受体介导的突触传递引起的能量需求增加引发的线粒体活性增加介导的反应一致。GABAA受体拮抗剂印防己毒素没有显着影响诱发反应。同样，外源性应用乙醇，在已知的浓度增加GABAA受体介导的突触传递浦肯野细胞，没有修改峰响应。这些观察结果表明，黄素蛋白自发荧光成像可能是有用的，以评估小脑切片中的突触传递和神经元代谢之间的耦合。
Flavoprotein autofluorescence signals attributed to neuronal metabolism have been used to assess synaptic function. Here, we characterized flavoprotein autofluorescence responses in the molecular layer of rat cerebellar slices. High frequency stimulation elicited a transient fluorescence increase (peak phase) that was followed by a longer-lasting fluorescence decrease (valley phase). The peak phase was restricted to the molecular layer, whereas the valley phase extended into the Purkinje cell layer and a portion of the granule cell layer. Responses were abolished by either the Na+ channel antagonist, tetrodotoxin, or a combination of the AMPA receptor antagonists, NBQX and GIKI-53655, and were also reduced by a flavoprotein inhibitor (diphenyleneiodonium). These findings are consistent with responses being mediated by an increase in mitochondrial activity triggered by increased energy demands evoked by AMPA receptor-mediated synaptic transmission. The GABAA receptor antagonist picrotoxin did not significantly influence evoked responses. Likewise, exogenous application of ethanol, at concentrations known to increase GABAA receptor-mediated synaptic transmission at Purkinje cells, did not modify peak responses. These observations indicate that flavoprotein autofluorescence imaging could be useful to assess the coupling between glutamatergic synaptic transmission and neuronal metabolism in cerebellar slices.
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影响因子：
1.2
作者：
Chery, Romain;L'Heureux, Barbara;Gurden, Hirac
通讯作者：
Gurden, Hirac
影响因子：
14.8
作者：
Theyel, Brian B.;Llano, Daniel A.;Sherman, S. Murray
通讯作者：
Sherman, S. Murray
乙醇通过增加大鼠小脑切片中 GABA 的释放来降低浦肯野神经元的兴奋性。
DOI：
10.1124/jpet.108.144865
发表时间：
2008-12
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Mameli M;Botta P;Zamudio PA;Zucca S;Valenzuela CF
通讯作者：
Valenzuela CF
影响因子：
2.5
作者：
Reinert, KC;Dunbar, RL;Ebner, TJ
通讯作者：
Ebner, TJ
体内小脑皮质黄素蛋白自发荧光的细胞和代谢起源
影响因子：
3.5
作者：
Reinert, Kenneth C.;Gao, Wangcai;Chen, Gang;Wang, Xinming;Peng, Yu-Ping;Ebner, Timothy J.
通讯作者：
Ebner, Timothy J.