Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells
Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells
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DOI：
10.1073/pnas.0709854105
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发表时间：
2008-06-17
影响因子：
11.1
通讯作者：
Jelinsky, Scott A.
中科院分区：
文献类型：
--
作者：
Johnston, Daniel S.;Wright, William W.;Jelinsky, Scott A.
哺乳动物精子发生是一个复杂的生物过程，发生在高度组织化的组织--生精上皮内。精原细胞、精母细胞和精子细胞的协调成熟表明，在这些细胞及其邻近的体细胞支持细胞中存在精确的基因表达程序。这项研究的目的是确定执行这些程序的基因。用显微切割法分离周期第I、II-III、IV-V、VI、VIIa、b、VIIC、d、VIII、IX-XI、XII和XIII-XIV期大鼠生精管，而从酶分散的睾丸中分离出支持细胞、精原细胞和早期精母细胞、粗线期精母细胞和圆形精子细胞。使用大鼠基因组230 2.0阵列进行的微阵列分析确定了16,971个识别睾丸转录本的探针组，其中398个被鉴定为睾丸特异的。1,286个探针组的表达在两种细胞类型之间以及在周期的不同阶段被发现至少相差4倍。对这些探针组的路径和注释聚类分析预测，整个生物路径和过程在特定细胞中受到循环调控。其中重要的是细胞周期、DNA修复和胚胎神经元发育。综上所述，这些数据表明，阶段调控的基因表达是生精细胞和支持细胞普遍存在的基本特征。
Mammalian spermatogenesis is a complex biological process that occurs within a highly organized tissue, the seminiferous epithelium. The coordinated maturation of spermatogonia, spermatocytes, and spermatids suggests the existence of precise programs of gene expression in these cells and in their neighboring somatic Sertoli cells. The objective of this study was to identify the genes that execute these programs. Rat seminiferous tubules at stages I, II-III, IV-V, VI, VIIa,b, VIIc,d, VIII, IX-XI, XII, and XIII-XIV of the cycle were isolated by microdissection, whereas Sertoli cells, spermatogonia plus early spermatocytes, pachytene spermatocytes, and round spermatids were purified from enzymatically dispersed testes. Microarray analysis by using Rat Genome 230 2.0 arrays identified 16,971 probe sets that recognized testicular transcripts, and 398 of these were identified as testis-specific. Expression of 1,286 probe sets were found to differ at least 4-fold between two cell types and also across the stages of the cycle. Pathway and annotated cluster analyses of those probe sets predicted that entire biological pathways and processes are regulated cyclically in specific cells. Important among these are the cell cycle, DNA repair, and embryonic neuron development. Taken together, these data indicate that stage-regulated gene expression is a widespread and fundamental characteristic of spermatogenic cells and Sertoli cells.