Defense Responses in Rice Induced by Silicon Amendment against Infestation by the Leaf Folder Cnaphalocrocis medinalis.
Defense Responses in Rice Induced by Silicon Amendment against Infestation by the Leaf Folder Cnaphalocrocis medinalis.
复制标题
硅改良剂诱导的水稻对稻纵卷叶螟侵染的防御反应
DOI：
10.1371/journal.pone.0153918
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hou M
中科院分区：
文献类型：
--
作者：
Han Y;Li P;Gong S;Yang L;Wen L;Hou M
植物施硅可增强对植食性害虫的抗性。在本研究中，在亚洲国家的最具破坏性的水稻害虫之一，稻纵卷叶螟，稻纵卷叶螟（Guenée）的Si添加植物的抗性增强的生理和细胞学机制进行了研究。防御相关酶，超氧化物歧化酶，过氧化物酶，过氧化氢酶，苯丙氨酸解氨酶，多酚氧化酶的活性，丙二醛和可溶性蛋白质的浓度测定在水稻植株与或不与叶夹感染和硅修正剂在0.32克硅/公斤土壤。硅修正案显着降低叶蝉幼虫的生存。单独施硅不影响水稻叶片防御相关酶活性和丙二醛含量。与叶夹感染，防御相关酶的活性增加，丙二醛浓度降低与硅修正植物。可溶性蛋白质含量增加与Si添加时，植物没有被感染，但减少更多的被感染的植物与Si修正案比那些没有Si添加。无论叶夹侵扰，硅修正案显着增加叶片硅含量，通过增加二氧化硅细胞的数量和宽度。结果表明，硅通过启动水稻的取食胁迫防御系统、降低叶片可溶性蛋白质含量和细胞硅化等途径增强水稻对稻纵卷叶螟的抗性。
Silicon (Si) amendment to plants can confer enhanced resistance to herbivores. In the present study, the physiological and cytological mechanisms underlying the enhanced resistance of plants with Si addition were investigated for one of the most destructive rice pests in Asian countries, the rice leaf folder, Cnaphalocrocis medinalis (Guenée). Activities of defense-related enzymes, superoxide dismutase, peroxidase, catalase, phenylalanine ammonia-lyase, and polyphenol oxidase, and concentrations of malondialdehyde and soluble protein in leaves were measured in rice plants with or without leaf folder infestation and with or without Si amendment at 0.32 g Si/kg soil. Silicon amendment significantly reduced leaf folder larval survival. Silicon addition alone did not change activities of defense-related enzymes and malondialdehyde concentration in rice leaves. With leaf folder infestation, activities of the defense-related enzymes increased and malondialdehyde concentration decreased in plants amended with Si. Soluble protein content increased with Si addition when the plants were not infested, but was reduced more in the infested plants with Si amendment than in those without Si addition. Regardless of leaf folder infestation, Si amendment significantly increased leaf Si content through increases in the number and width of silica cells. Our results show that Si addition enhances rice resistance to the leaf folder through priming the feeding stress defense system, reduction in soluble protein content and cell silicification of rice leaves.