Induction of phytochelatins and antioxidant defence system in Brassica juncea and Vigna radiata in response to chromium treatments
Induction of phytochelatins and antioxidant defence system in Brassica juncea and Vigna radiata in response to chromium treatments
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DOI：
10.1007/s10725-010-9454-0
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发表时间：
2010-05-01
影响因子：
4.2
通讯作者：
Iqbal, Muhammad
中科院分区：
文献类型：
--
作者：
Diwan, Hema;Khan, Ishrat;Iqbal, Muhammad
研究了不同Cr水平（0、50、100、200亩M Cr）下水培芥菜（Brassica juncea）和绿豆（Vigna radiata）的生长性能、Cr积累势、抗氧化防御系统和植物螯合素（PCs）的诱导作用。芥菜根和地上部Cr积累量分别比辐射菜高2倍和3倍。与juncea相比，辐射弧菌对Cr特别敏感，表现为Cr毒性症状的严重程度和发展以及生长下降。测定了植物对金属的初级和次级解毒反应中PC和抗氧化防御系统酶的诱导情况。两种植物均有PC和抗氧化防御系统酶的诱导作用。在不同Cr处理下，两种植株的根和茎部均有显著的pc诱导。在不同Cr处理下，芥菜茎部超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）活性均显著高于辐射菜。在Cr胁迫下诱导pc和抗氧化防御系统，表明pc和抗氧化剂在芥菜中积累了对Cr的耐受性，从而表明该植物适合作为潜在的Cr修复剂之一。
Growth performance, chromium (Cr) accumulation potential and induction of antioxidative defence system and phytochelatins (PCs) were studied in hydroponically grown Brassica juncea (Indian mustard) and Vigna radiata (mungbean) at various levels of Cr treatments (0, 50, 100, 200 mu M Cr). B. juncea accumulated twofolds and threefolds higher Cr in root and shoot, respectively than in V. radiata. Compared to B. juncea, V. radiata was found to be particularly sensitive to Cr as observed by the severity and development of Cr toxicity symptoms and decreased growth. Induction of PC and enzymes of antioxidant defence system were monitored as plant's primary and secondary metal detoxifying responses, respectively. There was induction of PC and enzymes of antioxidant defence system in both the plants. PCs were induced significantly in roots and shoot of both the plants at all the levels of Cr treatments. Significantly higher activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) were observed in shoot of B. juncea than V. radiata at all the levels of Cr treatments. Induction of PCs along with antioxidant defence system in response to Cr stress suggests the cumulative role of PCs and antioxidants in conferring tolerance against accumulated Cr in B. juncea, and thereby signifies the suitability of this plant as one of the potential remediators of Cr.