Interleukin-6-induced tyrosine phosphorylation of phospholipase C-gamma1 in PC12 cells.
Interleukin-6-induced tyrosine phosphorylation of phospholipase C-gamma1 in PC12 cells.
复制标题
PC12 细胞中白细胞介素 6 诱导的磷脂酶 C-gamma1 酪氨酸磷酸化。
作者：
Young Han Lee;S. Bae;Jeong Kon Seo;I. Choi;S. Ryu;Pann Gill Suh
磷脂酶C（PLC）-γ 1在生长因子介导的信号转导途径中起着关键作用。本研究发现，白细胞介素-6（IL-6）可显著诱导嗜铬细胞瘤（PC 12）细胞突起生长。用IL-6刺激PC 12细胞导致PLC-γ 1以剂量和时间依赖性方式酪氨酸磷酸化。IL-6刺激也增加了磷脂酰肌醇4，5-二磷酸的水解。总肌醇磷酸的积累以及PLC-γ 1的酪氨酸磷酸化被蛋白激酶抑制剂如染料木素和星形孢菌素的预处理抑制。这些结果提示PLC-γ 1可能参与了IL-6诱导PC 12细胞分化的信号转导通路。
Phospholipase C (PLC)-gamma1 plays a pivotal role in the signal transduction pathway mediated by growth factors. In this study, we found that neurite outgrowth of pheochromocytoma (PC12) cells was significantly induced by interleukin-6 (IL-6). Stimulation of PC12 cells with IL-6 led to tyrosine phosphorylation of PLC-gamma1 in a dose- and time-dependent manner. IL-6 stimulation also increased the hydrolysis of phosphatidylinositol 4,5-bisphosphate. Accumulation of total inositol phosphate as well as tyrosine phosphorylation of PLC-gamma1 was inhibited by the pretreatment of protein kinase inhibitors such as genistein and staurosporine. These results suggest that PLC-gamma1 may be involved in the signal transduction pathway of IL-6-induced PC12 cell differentiation.