POLYMYXIN-B AND POLYMYXIN-B NONAPEPTIDE ALTER CYTOPLASMIC MEMBRANE-PERMEABILITY IN ESCHERICHIA-COLI
POLYMYXIN-B AND POLYMYXIN-B NONAPEPTIDE ALTER CYTOPLASMIC MEMBRANE-PERMEABILITY IN ESCHERICHIA-COLI
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DOI：
10.1093/jac/18.5.557
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发表时间：
1986-11-01
影响因子：
5.2
通讯作者：
CHOPRA, I
中科院分区：
文献类型：
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作者：
DIXON, RA;CHOPRA, I
研究了多粘菌素B和多粘菌素B九肽(PMBN)对大肠杆菌细胞质膜通透性的影响。这两种化合物都会导致大肠杆菌中游离氨基酸、尿嘧啶和K+的损失。多粘菌素B促进的损失率是PMBN的1.5~2倍。虽然PMBN介导了低分子物质从大肠杆菌中的损失，但它并不杀菌。相反，多粘菌素B处理裂解并迅速丧失活力的大肠杆菌。我们认为，多粘菌素B的杀菌活性可能与其从细菌中释放细胞质蛋白的能力有关。
The effects of polymyxin B and polymyxin B nonapeptide (PMBN) on the permeability of the Escherichia coli cytoplasmic membrane were investigated. Both compounds caused loss of free amino acids, uracil and K+ from E. coli. The rates of loss promoted by polymyxin B were one and a half to two-fold greater than those caused by PMBN. Although PMBN mediated loss of low molecular weight substances from E. coli, it was not bactericidal. In contrast, polymyxin B treated E. coli lysed and rapidly lost viability. We suggest that the bactericidal activity of polymyxin B may be related to its previously reported ability to release cytoplasmic proteins from bacteria.