Morphological analysis of the pancreas and liver in diabetic KK-Ay mice treated with zinc and oxovanadium complexes.
Morphological analysis of the pancreas and liver in diabetic KK-Ay mice treated with zinc and oxovanadium complexes.
复制标题
用锌和氧钒复合物治疗的糖尿病 KK-Ay 小鼠的胰腺和肝脏的形态学分析。
DOI：
10.1039/c4mt00087k
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发表时间：
2014
期刊：
影响因子：
3.4
通讯作者：
Airo Tsubura and Hiroyuki Yasui
中科院分区：
文献类型：
--
作者：
Takayasu Moroki;Yutaka Yoshikawa;Katsuhiko Yoshizawa;Airo Tsubura and Hiroyuki Yasui
生物金属，如锌（Zn2+），钒，铜，钴和镁离子与糖尿病治疗之间的关系已经被认识了几年。特别是，抗糖尿病活性的Zn 2+和氧钒（VO2+）配合物已被测量使用生物化学方法。本研究用双（1-氧-2-吡啶硫醇基）锌（Zn（opt）2）和双（1-氧-2-吡啶硫醇基）钒（VO（opt）2）处理糖尿病小鼠KK-Aymice 4周，用生化和形态学方法评价这些金属配合物的抗糖尿病活性。此外，葡萄糖酸锌（Zn（glc）2）和双（乙基麦芽酚）VO2+（VO（emal）2）用作参比化合物。胰岛细胞较小，并且与未治疗的KK-Aymice相比，Zn（opt）2治疗的小鼠中的胰岛细胞面积比有降低的趋势。此外，血浆胰岛素浓度显著降低至未给药KK-Aymice中胰岛素浓度的27.2%。这些结果表明，Zn（opt）2管理提供形态和生化改善高胰岛素血症。与此相反，在接受Zn（glc）2和VO2+复合物的小鼠中，胰岛细胞大小和胰岛细胞面积比与未治疗的对照组没有差异。Zn（opt）2和VO（opt）2处理的小鼠在肝脏中表现出显著较低的脂肪沉积和脂肪沉积面积比（分别为未处理的KK-Aymice的63.6%和65.8%），与未处理的KK-Aymice中观察到的那些相比。Zn（opt）2或VO（opt）2处理对胰腺和肝脏形态改善的差异可能是由于Zn~（2+）和VO~（2+）复合物在KK-Aymice不同器官中作用部位的差异。结论：Zn（opt）2的抗糖尿病作用优于VO（opt）2，这是由于Zn（opt）2对肝脏和胰腺的形态学参数有较大改善。
The relationship between biometals, such as zinc (Zn2+), vanadium, copper, cobalt, and magnesium ions, and diabetes therapy has been recognized for several years. In particular, the antidiabetic activities of Zn2+and oxovanadium (VO2+) complexes have been measured using biochemical approaches. In the present study, diabetic KK-Aymice were treated with bis(1-oxy-2-pyridine-thiolato)Zn2+(Zn(opt)2) and bis(1-oxy-2-pyridine-thiolato)VO2+(VO(opt)2) for 4 weeks, and the antidiabetic activities of these metal complexes were evaluated using biochemical and morphological methods. Additionally, zinc gluconate (Zn(glc)2) and bis(ethylmaltolato)VO2+(VO(emal)2) were used as reference compounds. Pancreatic islet cells were smaller, and there was a tendency towards a lower islet cell area ratio in Zn(opt)2-treated mice compared with nontreated KK-Aymice. Furthermore, plasma insulin concentrations were significantly reduced to 27.2% of insulin concentrations in nontreated KK-Aymice. These results suggest that Zn(opt)2administration provides morphological and biochemical improvements in hyperinsulinaemia. In contrast, in mice that received Zn(glc)2and VO2+complexes, the islet cell size and islet cell area ratio did not differ from those in nontreated controls. Zn(opt)2- and VO(opt)2-treated mice exhibited significantly lower fat deposition and fat deposition area ratio in the liver (63.6% and 65.8% of nontreated KK-Aymice, respectively) compared to those observed in nontreated KK-Aymice. The differences in morphological improvements of the pancreas and liver owing to Zn(opt)2or VO(opt)2treatment may be explained by differences in the sites of actions of Zn2+and VO2+complexes in different organs in KK-Aymice. In conclusion, Zn(opt)2exhibited superior antidiabetic effects over those of VO(opt)2, and this was owing to greater amelioration of the morphological parameters of the liver and pancreas.