Integrated metabolomic and transcriptomic strategies to understand the effects of dark stress on tea callus flavonoid biosynthesis
Integrated metabolomic and transcriptomic strategies to understand the effects of dark stress on tea callus flavonoid biosynthesis
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综合代谢组学和转录组学策略来了解黑暗胁迫对茶愈伤组织类黄酮生物合成的影响
DOI：
10.1016/j.plaphy.2020.07.048
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发表时间：
2020-10-01
影响因子：
6.5
通讯作者：
Chen, Zhongzheng
中科院分区：
文献类型：
--
作者：
Shi, Jing;Zhang, Xue;Chen, Zhongzheng
类黄酮的生物合成是茶树重要的次生代谢过程。其代谢受多种环境因素的影响，尤其是光。遮荫，又称暗胁迫(DS)，通常用于栽培过程中，通过影响茶叶中黄酮类物质的积累来改善茶叶品质。以茶树愈伤组织为材料，在12h光照/12h暗周期(A)和完全黑暗(B)条件下培养30d，对茶愈伤组织进行代谢组学和转录组学分析，以探讨DS条件下茶树类黄酮生物合成的分子机制。共鉴定出161个差异代谢产物(DMP)和3592个差异表达基因(DEG)。茶愈伤组织中表没食子儿茶素没食子酸酯、花青素3-O-葡萄糖苷等主要类黄酮含量以及儿茶素、花青素和原花青素总量均显著降低。同时确定了CsPAL、Cs4CL、CsCHS、CsFLS、CsDFR、CsANS、CsLAR、CsANR和CsUFGT等9个基因参与了类黄酮的生物合成。此外，CsMYBT1和CsMYBT2等2个转录因子在类黄酮生物合成中起着关键的调控作用。这些结果有助于我们进一步了解茶树类黄酮代谢的潜在分子机制。
Flavonoid biosynthesis is a crucial secondary metabolism process for tea plants. Its metabolism is affected by multiple environmental factors, especially light. Shade, also known as dark stress (DS), is generally used during cultivation to improve tea quality by influencing the flavonoid accumulation. To explore the molecular mechanisms of flavonoid biosynthesis under DS, metabolomics and transcriptomics (METR) analyses were performed in tea callus via culturing the plants in vitro using 12 h light/12 h dark cycles (A) or completely dark (B) conditions for 30 days. In total, 161 differential metabolic products (DMPs) and 3592 differential expression genes (DEGs) were identified. The major flavonoids including epicatechin gallate, catechin gallate, gallocatechincatechin, cyanidin 3-O-glucoside and the total of catechin, anthocyanin and proanthocyanidin contents were all remarkably down-regulated in tea callus under DS. Meanwhile, 9 genes including CsPAL, Cs4CL, CsCHS, CsFLS, CsDFR, CsANS, CsLAR, CsANR, and CsUFGT determined to be responsible for the flavonoid biosynthesis. In addition, 2 transcription factors (TFs) including CsMYBT1 and CsMYBT2 verified to play key role in regulation the flavonoid biosynthesis. These results helped us further understand the underlying molecular mechanism of flavonoid metabolism in tea plants.