Beta-blocker therapy preserves normal splenic T-lymphocyte numbers reduced in proportion to sepsis severity in a sepsis model
Beta-blocker therapy preserves normal splenic T-lymphocyte numbers reduced in proportion to sepsis severity in a sepsis model
复制标题
β-受体阻滞剂治疗可保留正常的脾 T 淋巴细胞数量，在脓毒症模型中，其数量与脓毒症严重程度成比例减少
DOI：
10.1155/2019/8157482
复制
发表时间：
2019
影响因子：
1.7
通讯作者：
Morisaki H
中科院分区：
文献类型：
--
作者：
Suzuki T;Inoue K;Igarashi T;Kato J;Nagata H;Yamada T;Minamishima S;Morisaki H
淋巴细胞死亡有助于脓毒症诱导的免疫抑制，导致预后不良。本研究检查了败血症严重程度和β受体阻滞剂治疗是否会影响败血症小鼠模型中T淋巴细胞死亡的程度。在第一项对照研究中，将20只动物分配至4组：假手术对照组（C组，n= 5）和在3个不同部位进行盲肠结扎和穿刺（CLP）的3个组：盲肠近端、中段和远端（分别为CLP-P、CLP-M和CLP-D组;每组n= 5）。CLP后24 h，在全麻下切除脾脏，流式细胞术检测正常脾T淋巴细胞总数和凋亡T淋巴细胞百分比。在第二项实验研究中，检查了β受体阻滞剂艾司洛尔在CLP-P中的作用（CLP-PE组与CLP-P组;每组n= 5）。每只小鼠正常脾T淋巴细胞总数与CLP严重程度成比例显著降低（C组，18.6 × 106（15 × 106-23.6 × 106）; CLP‐D组，9.2 × 106（8.8 × 106-9.8 × 106）; CLP‐M，6.7 × 106（6.3 × 106-7.0 × 106）; CLP‐P，5.3 × 106（5.1 × 106-6.8 × 106））。β受体阻滞剂治疗恢复了T淋巴细胞数量（CLP-PE组vs. CLP-P组; 6.94 ± 1.52 × 106 vs. 4.18± 1.71 × 106;p= 0.027）而不影响凋亡百分比。β受体阻滞剂治疗可通过正常脾T淋巴细胞保存改善脓毒症诱导的免疫抑制。
Lymphocyte cell death contributes to sepsis‐induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta‐blocker therapy could affect the degree of T‐lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated to 4 groups: control group with sham operation (group C,n= 5) and 3 groups with cecum ligation and puncture (CLP) performed at 3 different sites: proximal, middle, and distal cecum (groups CLP‐P, CLP‐M, and CLP‐D, respectively;n= 5 in each group). Their spleens were resected under general anesthesia 24 hours after CLP, and the total number of normal splenic T lymphocytes per mouse and the percentage of apoptotic T lymphocytes were evaluated using flow cytometry. In the second experimental study, the effect of the beta‐blocker esmolol was examined in CLP‐P (group CLP‐PE vs. CLP‐P;n= 5 in each group). The total normal splenic T‐lymphocyte numbers per mouse significantly decreased in proportion to CLP severity (group C, 18.6 × 106(15 × 106–23.6 × 106); CLP‐D, 9.2 × 106(8.8 × 106–9.8 × 106); CLP‐M, 6.7 × 106(6.3 × 106–7.0 × 106); and CLP‐P, 5.3 × 106(5.1 × 106–6.8 × 106)). Beta‐blocker therapy restored T‐lymphocyte numbers (group CLP‐PE vs. CLP‐P; 6.94 ± 1.52 × 106vs. 4.18 ± 1.71 × 106;p= 0.027) without affecting apoptosis percentage. Beta‐blocker therapy might improve sepsis‐induced immunosuppression via normal splenic T‐lymphocyte preservation.