One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.
One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.
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DOI：
10.1038/srep21953
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发表时间：
2016-02-24
影响因子：
4.6
通讯作者：
Kues WA
中科院分区：
文献类型：
--
作者：
Garrels W;Talluri TR;Apfelbaum R;Carratalá YP;Bosch P;Pötzsch K;Grueso E;Ivics Z;Kues WA
转基因牛对于开发新的生物医学模型和提高对人畜共患病病理生理学的理解具有重要意义。然而，基于体细胞核移植的基因组编辑和基因工程的总体效率较低。在这里，我们建立了一个高效的一步多重基因转移系统到牛的基因组。
Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.