Genetic diversity and structure of Oncomelania hupensis hupensis in two eco-epidemiological settings as revealed by the mitochondrial COX1 gene sequences
Genetic diversity and structure of Oncomelania hupensis hupensis in two eco-epidemiological settings as revealed by the mitochondrial COX1 gene sequences
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DOI：
10.1007/s11033-021-06907-8
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发表时间：
2021-11
影响因子：
2.8
通讯作者：
Jie-Ying Zhang;Man-Man Gu-Man;Qiu-Fu Yu;Mengtao Sun;Hui-Ying Zou;Zhichun Zhou;D. Lu
中科院分区：
文献类型：
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作者：
Jie-Ying Zhang;Man-Man Gu-Man;Qiu-Fu Yu;Mengtao Sun;Hui-Ying Zou;Zhichun Zhou;D. Lu
研究背景湖北钉螺是日本血吸虫唯一的中间宿主，是我国血吸虫病的主要病原，具有重要的医学和兽医学意义。虽然已经取得了巨大的进展，但仍有约20.6亿平方米的潜在蜗牛栖息地未得到充分研究。每年的洪水可能会进一步增加这一面积。因此，在这些地区的螺的群体遗传学的理解可能是有用的，为今后的监测和控制activities.Methods和resultsWe抽样钉螺从和县（HX），枞阳（ZY）和石台（ST）在安徽（血吸虫病传播控制），和横塘（HT），太仓（TC），东三（DS）和西三（XS）在江苏（血吸虫病传播中断），安徽的下游。ST、DS、XS为丘陵山区，HX、ZY、TC、HT为湖沼区。对线粒体细胞色素c氧化酶亚基I基因进行测序，分析115只蜗牛，鉴定出29种单倍型。我们观察到56（8.72%）多态性位点，包括51个转换，4个颠换和一个多重突变的变化。总的单倍型和核苷酸多样性分别为0.899和0.01569。安徽钉螺群体的遗传多样性高于江苏钉螺群体。总变异的73.32%分布在点间，26.68%分布在点内。根据网络和系统发育分析中的生态流行病学设置，蜗牛被显着分离。japonicum，以及今后的软体动物宿主监测和控制活动。
BackgroundOncomelania hupensis hupensisis the only intermediate host ofSchistosoma japonicum, the causative agent of schistosomiasis in China and is therefore of significant medical and veterinary health importance. Although tremendous progress has been achieved, there remains an understudied area of approximately 2.06 billion m2of potential snail habitats. This area could be further increased by annual flooding. Therefore, an understanding of population genetics of snails in these areas may be useful for future monitoring and control activities.Methods and resultsWe sampled snails from Hexian (HX), Zongyang (ZY) and Shitai (ST) in Anhui (schistosomiasis transmission control), and from Hengtang (HT), Taicang (TC), Dongsan (DS) and Xisan (XS) in Jiangsu (schistosomiasis transmission interrupted), downstream of Anhui. ST, DS and XS are classified as hilly and mountainous areas, and HX, ZY, TC and HT as lake and marshland areas. The mitochondrial cytochrome c oxidase subunit I gene were sequenced.Out of 115 snails analyzed, 29 haplotypes were identified. We observed 56 (8.72%) polymorphic sites consisting of 51 transitions, four transversions and one multiple mutational change. The overall haplotype and nucleotide diversity were 0.899 and 0.01569, respectively. Snail populations in Anhui had higher genetic diversity than in Jiangsu. 73.32% of total variation was distributed among sites and 26.68% within sites. Snails were significantly separated according to eco-epidemiological settings in both network and phylogenetic analyses.ConclusionOur results could provide important guidance towards assessing coevolutionary interactions of snails withS. japonicum, as well as for future molluscan host monitoring and control activities.