6th World Congress of Biomechanics (WCB 2010). August 1-6, 2010 Singapore
6th World Congress of Biomechanics (WCB 2010). August 1-6, 2010 Singapore
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第六届世界生物力学大会（WCB 2010）。
DOI：
10.1007/978-3-642-14515-5_289
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Nikolaev N
中科院分区：
文献类型：
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作者：
Nikolaev N
这项工作描述和测量了由于运输过程中的动态机械力对包装为立即临床使用的细胞造成的损害。了解这一点对于悬浮运输的细胞和对冻融循环敏感的细胞类型(如干细胞)非常重要。放置在冷室中的振动振动器系统模拟了冷容器中的细胞运输，其中温度可以保持在2-8摄氏度之间。来自已建立的细胞库的细胞供应给出了受控的细胞源和传代编号。将密度为106cell/ml的细胞悬液1ml插入2ml的小瓶中，头部间距为10 mm，安装在摇床上。对照小瓶储存在相同的条件下。摇床后，测量活细胞数、完整细胞总数(活细胞加上完好但不活的细胞)和活细胞与对照组的比率。细胞自动计数系统CEDEX采用台盼蓝排除法评估细胞的活性，采用不同的方法模拟道路振动来估计显著影响细胞活性的振动范围。最初的实验施加了重复的20ms半正弦脉冲，频率为1赫兹，类似于道路颠簸和跌落造成的冲击，细胞存活率没有显著变化。然而，使用类似随机振动频率的混合物进行的第二个实验表明，只有0.1%的初始细胞存活，活细胞与细胞总数的比率也很低，为2.1%。第三个实验使用10-5000赫兹的单个频率，并控制了幅度和加速度，澄清了在10-100赫兹的频率范围内的振动引起了显著的细胞死亡，并证实了在运输中遇到的那些幅度的加速的生存能力下降。
This work characterizes and measures damage, due to dynamic mechanical forces during transportation, to cells packaged for immediate clinical use. Understanding this is important for cells transported in suspension and for cell types sensitive to freeze-thawing cycles such as stem cells.A vibrating shaker system placed in a cold room emulated cell transportation in cold containers where the temperature can be maintained at between 2-8oC. Cell supply from an established cell bank gave a controlled cell source and passage number. 1ml suspensions of cells with a density of 106cell/ml were inserted in 2ml vials with 10mm head space and were mounted on the shaker. Control vials were stored in the same conditions. After shaking, the number of viable cells, the total number of intact cells (viable cells plus those that are intact but not alive) and the ratio of viable cells to those in the control were measured. An automated cell counting system, the Cedex, was used to evaluate the viability of cells using the trypan blue exclusion method.Different approaches to mimicking road vibrations were employed to estimate ranges of vibrations which significantly affected the viability of cells. The initial experiment imposed repeating 20ms half sine shock pulses at 1Hz, similar to those caused by road bumps and drops, with no significant change in cell viability. A second experiment using a mixture of random vibration like frequencies however showed that only 0.1% of the initial cells survived and that the ratio between the viable and total number of cells was also low at 2.1%. A third experiment using individual frequencies from 10-5000Hz with controlled amplitude and acceleration clarified that significant cell death is caused by vibrations with frequencies in the range 10-100Hz and confirmed a decrease of viability for accelerations of the magnitude of those encountered in transport.