MicroRNA profiling: approaches and considerations.
MicroRNA profiling: approaches and considerations.
复制标题
microRNA分析：方法和考虑因素。
DOI：
10.1038/nrg3198
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发表时间：
2012-04-18
期刊：
影响因子：
--
通讯作者：
Tewari M
中科院分区：
文献类型：
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作者：
Pritchard CC;Cheng HH;Tewari M
MicroRNAs(MiRNAs)是一种小RNA(~22nT长)，在正常的生理和疾病环境中，它在转录后调控着广泛的生物体中数千个基因的表达。MiRNA表达谱越来越受欢迎，因为miRNAs作为基因表达网络中的关键调节因子，可以影响许多生物过程，也显示出作为疾病生物标志物的前景。技术进步使miRNA分析的各种平台得以开发，了解不同方法的优点和缺陷有助于有效地将miRNA分析用于不同的应用。我们在这里回顾了开展和解释miRNA图谱研究结果的主要考虑因素，以及miRNA图谱的当前和新兴应用。
MicroRNAs (miRNAs) are small RNAs (~22 nt long) that post-transcriptionally regulate the expression of thousands of genes in a broad range of organisms, in both normal physiologic and disease contexts. MiRNA expression profiling is gaining popularity because miRNAs, as key regulators in gene expression networks, can influence many biological processes and have also shown promise as biomarkers for disease. Technological advances have enabled the development of various platforms for miRNA profiling, and an understanding of the strengths and pitfalls of different approaches can aid in the effective use of miRNA profiling for diverse applications. We review here the major considerations for carrying out and interpreting results of miRNA profiling studies, as well as current and emerging applications of miRNA profiling.