3′ UTR signals necessary for expression of the Plasmodium gallinaceum ookinete protein, Pgs28, share similarities with those of yeast and plants
3′ UTR signals necessary for expression of the Plasmodium gallinaceum ookinete protein, Pgs28, share similarities with those of yeast and plants
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DOI：
10.1016/j.molbiopara.2004.06.005
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发表时间：
2004-10-01
影响因子：
1.5
通讯作者：
Golightly, LM
中科院分区：
文献类型：
--
作者：
Cann, H;Brown, SV;Golightly, LM
在后生动物发育过程中，3' UTR 信号介导基因表达的时间和地点。对于原生动物疟原虫寄生虫来说，蚊子中肠中的配子形成动合子是一个类似的发育过程。先前对 Pgs28（鸡疟原虫动合子的主要表面蛋白）表达所需的 3'UTR 信号的研究表明，3'UTR T 丰富区域和含有 ATTAAA 真核多聚腺苷酸共有基序的 DNA 序列对其表达是必要的。在后生动物发育过程中，富含 U 的元件可能与真核多聚腺苷酸共有信号一起发挥作用，以介导发育蛋白的表达。为了确定推定的疟原虫元件是否是 Pgs28 表达的介质，在质粒构建体中对这些核苷酸序列进行了突变。通过有性阶段鸡疟原虫寄生虫的瞬时基因转染来测试突变对 Pgs28 表达的影响。这些研究表明，ATTAAA 基序的两种不同突变会改变高等真核生物和酵母中的基因表达，但不会改变 Pgs28 的表达。然而，富含U的元件、相邻核苷酸UUUACAAAAUUGUUUUAACU和下游核苷酸UAAUUAAAA能够不同程度地介导表达。这些元素的组织和重叠功能似乎更类似于酵母或植物，而不是后生动物。 (C) 2004 Elsevier B.V. 保留所有权利。
During metazoan development, 3' UTR signals mediate the time and place of gene expression. For protozoan Plasmodium parasites, the formation of ookinetes from gametes in the mosquito midgut is an analogous developmental process. Previous studies of the 3' UTR signals necessary for expression of Pgs28, the major surface protein of Plasmodium gallinaceum ookinetes, suggested that a 3' UTR T-rich region and DNA sequences containing an ATTAAA eukaryotic polyadenylation consensus motif were necessary for its expression. During metazoan development, U-rich elements may function in conjunction with eukaryotic polyadenylation consensus signals to mediate developmental protein expression. To define whether the putative Plasmodium elements were mediators of Pgs28 expression mutations of these nucleotide sequences were made in plasmid constructs. The effect of the mutations on Pgs28 expression was tested by the transient gene transfection of sexual stage P. gallinaceum parasites. These studies reveal that two different mutations of the ATTAAA motif, which alter gene expression in higher eukaryotes and yeast, do not alter the expression of Pgs28. However, the U-rich element, adjacent nucleotides UUUACAAAAUUGUUUUAACU and downstream nucleotides UAUAUAAAA are able to mediate expression to varying degrees. The organization and overlapping function of these elements appears to more closely resemble that of yeasts or plants than those of metazoans. (C) 2004 Elsevier B.V. All rights reserved.