Application of a freeze-thaw technique to studies of radiation-induced cell transformation.
Application of a freeze-thaw technique to studies of radiation-induced cell transformation.
复制标题
冻融技术在辐射诱导细胞转化研究中的应用。
DOI：
10.1080/09553008814552251
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发表时间：
1988
影响因子：
2.6
通讯作者：
Redpath,JL
中科院分区：
文献类型：
--
作者：
Sun,C;Redpath,JL
In vitro cell systems are widely used in the study of mechanisms of neoplastic transformation by both physical and chemical agents. Such studies are invariably labour intensive, involve large numbers of tissue culture flasks and can occupy incubator space for long periods of time (up to 6 weeks). In a situation where the treatment of the cells involves access to a facility which is not readily available (eg because of location at a site remote from the laboratory), it is desirable to minimize the number of trips to that facility, yet maximize the value of those trips. One approach to such an issue is to give as many treatments as possible on a single trip to the remote facility and then store the treated cells as frozen suspensions in liquid N 2 for later retrieval for assay as the necessary incubator space becomes available. The feasibility of this approach is probably cell line dependent and in this technical note we report the results of experiments designed to test this idea in a cell system that we have recently developed for the study of radiation-induced neoplastic transformation (Redpath et al., 1987, Sun et al. 1988). The stimulus for this study was our desire to investigate the effects of high LET radiation at different dose-rates, which required travel to a remote site. A similar approach was recently described in which the aim was to freeze down synchronous mitotic and G1 cells for later use (Borrelli et al. 1987). However, in this latter study, a totally different freezing solution was employed from that used by us in this work. The cell system, culture conditions and assay used have been described previously (Redpath et al. 1987, Sun et al. 1988). The cells were human hybrid cells (HeLa x skin fibroblasts) and the cell line was designated as CGLI. The end-point was the radiation-induced expression of a tumour-associated antigen for which we have monoclonal antibody. We could therefore detect the foci of neoplasticallytransformed cells using immunoperoxidase staining. The experimental protocol is typical of that used in our previous work. CGLI cells are plated into 25 cm2 tissue culture flasks (T-25) at a density of 3 x 105 per flask. Two days later, this culture will have reached a confluent density of 10 6 cells per flask. At this time, the cells are irradiated and held at confluence for 6 hours at 37 C prior to the preparation of a cell suspension for subsequent dilution and plating for assay for survival and transformation. Immunoperoxidase staining was carried out 21 days after post-irradiation plating. In the experiments reported here we irradiated CGLI cells (10 6 per T-25 flask) with 7 Gy of gamma rays. Following 6 hours of incubation at 37 C, the cells were
伽马辐射对人类杂交细胞的肿瘤转化：定量测定。
影响因子：
3.4
作者：
Redpath,JL;Sun,C;Colman,M;Stanbridge,EJ
通讯作者：
Stanbridge,EJ
一种冷冻同步有丝分裂细胞和 G1 细胞的方法。
影响因子：
3.7
作者：
Borrelli,MJ;Mackey,MA;Dewey,WC
通讯作者：
Dewey,WC
关于人类细胞杂种中肿瘤特异性抗原的辐射诱导表达的进一步研究。
影响因子：
3.4
作者：
Sun,C;Redpath,JL;Colman,M;Stanbridge,EJ
通讯作者：
Stanbridge,EJ