Apoptosis of human retina and retinal pigment cells induced by human cytomegalovirus infection
Apoptosis of human retina and retinal pigment cells induced by human cytomegalovirus infection
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DOI：
10.1159/000048332
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发表时间：
2002-03-01
影响因子：
2.1
通讯作者：
Hsu, WM
中科院分区：
文献类型：
--
作者：
Chiou, SH;Liu, JH;Hsu, WM
人巨细胞病毒视网膜炎是免疫功能低下患者和艾滋病患者最常见的眼部机会性感染。如果不治疗，它往往会导致失明。关于人类巨细胞病毒感染如何导致艾滋病患者视网膜病变和视力损害的问题仍未得到解决，为了回答这个问题，我们利用末端脱氧核苷酸转移酶介导的脱氧尿嘧啶核苷缺口末端标记技术，检测了与艾滋病患者无HCMV视网膜炎的患者相比，感染了HCMV的艾滋病患者视网膜中相同部位的凋亡细胞。体外研究还发现，HCMV感染可通过激活caspase3和聚腺苷二磷酸核糖聚合酶途径诱导人视网膜色素上皮细胞发生凋亡。这些结果有力地表明，巨细胞病毒诱导的细胞凋亡在介导感染的人视网膜和视网膜色素上皮细胞死亡以造成严重视力损害中起着基础性作用。版权所有(C)2002 S.Karger AG，巴塞尔。
Human cytomegalovirus (HCMV) retinitis is the most common ocular opportunistic infection in immunocompromised patients and AIDS. It often leads to blindness if left untreated. The question as to how HCMV infection causes retinal pathogenesis and visual destruction in AIDS patients remains unresolved, To answer the question, by using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay, we detected the significant signals of apoptotic cells at the same sites in the HCMV-infected retina of AIDS patients as compared to AIDS patients without HCMV retinitis. In vitro study also revealed apoptosis induced by HCMV infection in human retinal pigment epithelium cells, mediated by activation of caspase 3 and poly(ADP-ribose) polymerase pathway. These results strongly suggest the fundamental role of HCMV-induced apoptosis in mediating cell death in infected human retina and retinal pigment epithelium cells to make severe visual impairment. Copyright (C) 2002 S. Karger AG, Basel.