Identification of CD4 and H-2Kd-restricted cytotoxic T lymphocyte epitopes on the human herpesvirus 6B glycoprotein Q1 protein
Identification of CD4 and H-2Kd-restricted cytotoxic T lymphocyte epitopes on the human herpesvirus 6B glycoprotein Q1 protein
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DOI：
10.1038/s41598-019-40372-5
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发表时间：
2019-03-07
影响因子：
4.6
通讯作者：
Mori, Yasuko
中科院分区：
文献类型：
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作者：
Nagamata, Satoshi;Aoshi, Taiki;Mori, Yasuko
人类疱疹病毒6 B（HHV-6 B）抗原表位的识别，由T细胞可以有助于潜在的疫苗和免疫治疗的发展。在此，我们通过使用编码BgQ 1的质粒DNA的体内电穿孔，跨越BgQ 1序列的重叠肽，用于定量γ干扰素的ELISPOT测定，鉴定了HHV-6 B（BgQ 1）的糖蛋白Q1上的CD 4（+）和H-2K（d）限制性CD 8（+）T细胞表位，BgQ 1是HHV-6 B病毒进入所必需的独特糖蛋白（IFN-γ）和基于计算机的T细胞表位预测程序。本研究在BALB/c小鼠中鉴定的CD 4（+）和CD 8（+）T细胞表位可以作为一个良好的动物模型系统用于开发T细胞应答、诱导HHV-6 B疫苗或免疫治疗。
The identification of Human herpesvirus 6B (HHV-6B) epitopes that are recognized by T-cells could contribute to the development of potential vaccines and immunotherapies. Here, we identified CD4(+) and H-2K(d)-restricted CD8(+) T-cell epitopes on the glycoprotein Q1 of HHV-6B (BgQ1), which is a unique glycoprotein and essential for HHV-6B viral entry, by using in vivo electroporation with a plasmid DNA encoding BgQ1, overlapping peptides spanning the BgQ1 sequence, ELISPOT assay for quantification of gamma interferon (IFN-gamma), and computer-based T-cell epitope prediction programs. The CD4(+) and CD8(+) T-cell epitopes identified in BALB/c mice in this study could be a good animal model system for use in the development of T-cell responses, inducing HHV-6B vaccines or immunotherapies.