Differential growth of human embryos in vitro: role of reactive oxygen species
Differential growth of human embryos in vitro: role of reactive oxygen species
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DOI：
10.1016/j.fertnstert.2004.02.121
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发表时间：
2004-09-01
影响因子：
6.7
通讯作者：
Agarwal, A
中科院分区：
文献类型：
--
作者：
Bedaiwy, MA;Falcone, T;Agarwal, A
目的：研究早期人类胚胎发育与受精后第一天（第 1 天 ROS）培养基中活性氧 (ROS) 水平的关系。设计：一项前瞻性研究。背景：在一所教学医院接受辅助生殖的患者。患者：接受传统 IVF（n = 104；115 个周期）和胞浆内单精子注射 (ICSI)（n = 91；96 个周期）的患者。受精和早期培养均在含有 5% 血清替代品补充剂的人输卵管液中进行。干预措施：过夜孵育后，使用鲁米诺作为探针，通过化学发光测定测量每个胚胎培养皿的中央孔（样品）和外孔（对照）中的第 1 天 ROS 水平。主要结果测量：记录每个周期第 3 天和第 5 天的受精率和胚胎质量。还比较了年龄、胎次和人口特征。结果：培养基中高日 I ROS 水平与 ICSI 中的低囊胚率、低受精率、低卵裂率和高胚胎破碎率相关，但与传统 IVF 无关。第 1 天培养基中的高 ROS 水平与 IVF 和 ICSI 周期中较低的妊娠率相关。结论：第 1 天培养基中产生的活性氧可能是早期胚胎生长的重要生化标志物。胚胎碎片增加和卵裂率减慢可能部分归因于 ICSI 周期中胚胎早期暴露于高 ROS 水平。在相同条件下孵育的 ICSI 胚胎的差异生长可能部分归因于这些胚胎周围培养基的 ROS 水平的差异。 (C) 2004 年，美国生殖医学会。
Objective: To examine the relationship of early human embryonic development with the level of reactive oxygen species (ROS) in the culture media on the first day (day 1 ROS) after insemination.Design: A prospective study.Setting: Patients undergoing assisted reproduction in a teaching hospital.Patient(s): Patients undergoing conventional IVF (n = 104; 115 cycles) and intracytoplasmic sperm injection (ICSI) (n = 91; 96 cycles) were included. Both fertilization and early cultures were performed in human tubal fluid with 5% serum substitute supplement.Intervention(s): Day 1 ROS levels in the central well (sample) and the outer well (control) of each embryo culture dish were measured after overnight incubation by chemiluminescence assay using luminol as the probe.Main Outcome Measure(s): Fertilization rate and embryo quality at day 3 and 5 were recorded for each cycle. Age, parity, and demographic features were also compared.Result(s): High day I ROS levels in culture media were associated with low blastocyst rate, low fertilization rate, low cleavage rate, and high embryonic fragmentation with ICSI but not with conventional IVF. High day 1 ROS levels in culture media were associated with lower pregnancy rates in both IVF and ICSI cycles.Conclusion(s): Reactive oxygen species generated in culture media by day 1 may be an important biochemical marker for early embryonic growth. Increased embryonic fragmentation and slow cleavage rate may be partially attributed to early exposure of embryos to high ROS levels in ICSI cycles. Differential growth of ICSI embryos incubated under identical conditions may be in part due to differences in ROS levels of the culture medium surrounding these embryos. (C) 2004 by American Society for Reproductive Medicine.