Volatilization of Mercury by Immobilized Bacteria (Klebsiella pneumoniae) in Different Support by Using Fluidized Bed Bioreactor

Volatilization of Mercury by Immobilized Bacteria (Klebsiella pneumoniae) in Different Support by Using Fluidized Bed Bioreactor
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流化床生物反应器在不同支持物下固定化细菌(肺炎克雷伯菌)的汞挥发

DOI:
10.1007/s002840010310
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发表时间:
2001
影响因子:
2.6
通讯作者:
M. Blaghen
M. Blaghen
中科院分区:
生物学4区
文献类型:
--
作者:
Y. Zeroual;A. Moutaouakkil;M. Blaghen

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从卡萨布兰卡的废水中分离到一株能将离子汞还原为金属汞的耐汞细菌肺炎克雷伯氏菌。该菌株对重金属(如汞2400 μM、铅8000 μM、银2400 μM和镉1000 μM)的最低抑制浓度较高。将该菌固定化于海藻酸钠、聚丙烯酰胺、蛭石和山毛榉中,并利用流化球生物反应器从汞污染的人工合成水中去除汞。固定化肺炎克雷伯氏菌细胞能有效挥发汞和解毒汞化合物。此外,汞挥发的效率比天然电池高得多。海藻酸钠微球包埋肺炎克雷伯氏菌时,其净化率和挥发率最高,分别为100%和89%。藻酸盐中的固定化细胞即使在10天后也持续挥发汞而不丧失活性。
Klebsiella pneumoniae, a mercury-resistant bacterial strain able to reduce ionic mercury to metallic mercury, was isolated from wastewater of Casablanca. This strain exhibits high minimal inhibition concentrations for heavy metals such as mercury 2400 μM, lead 8000 μM, silver 2400 μM, and cadmium 1000 μM. This bacterium was immobilized in alginate, polyacrylamide, vermiculite, and cooper beech and was used for removing mercury from a synthetic water polluted by mercury by using a fluidized bead bioreactor. Immobilized bacterial cells of Klebsiella pneumoniae could effectively volatilize mercury and detoxify mercury compounds. Moreover, the efficiency of mercury volatilization was much greater than with the native cells. The highest cleanup and volatilization rates were obtained when Klebsiella pneumoniae was entrapped in alginate beads, with a cleanup rate of 100% and a volatilization rate of 89%. Immobilized cells in alginate continuously volatilized mercury even after 10 days without loss of activity.
DOI: 10.1021/bi982680c
发表时间: 1999-03-23
期刊: BIOCHEMISTRY
影响因子: 2.9
作者:
Engst, S;Miller, SM
通讯作者: Miller, SM