The preventive effects of dexmedetomidine on endotoxin-induced exacerbated post-incisional pain in rats
The preventive effects of dexmedetomidine on endotoxin-induced exacerbated post-incisional pain in rats
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DOI：
10.1007/s00540-017-2374-7
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发表时间：
2017-05
影响因子：
2.8
通讯作者：
Daiki Yamanaka;T. Kawano;A. Nishigaki;Bun Aoyama;H. Tateiwa;Marie Shigematsu-Locatelli;F. Locatelli
中科院分区：
文献类型：
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作者：
Daiki Yamanaka;T. Kawano;A. Nishigaki;Bun Aoyama;H. Tateiwa;Marie Shigematsu-Locatelli;F. Locatelli
目的低度内毒素（脂多糖；LPS）暴露可能导致术后急性疼痛加剧。在本研究中，我们在大鼠切口痛模型中研究了术中给予右美托咪定 (DEX) 对 LPS 诱导的术后痛觉过敏的可能影响。方法将手术动物和假手术动物随机分为生理盐水治疗对照组、5.0 mg/kg LPS 治疗、10 µg/kg DEX 治疗和 5.0 mg/kg LPS + 10 µg/kg DEX 处理组。在手术动物中，在异氟烷麻醉下通过皮肤和筋膜切开一个 1 厘米长的足底切口。假手术大鼠仅被麻醉。所有治疗均在手术前 60 分钟通过单次腹膜内 (i.p.) 注射进行。在手术前一天（基线）和切口后 2 小时使用大鼠鬼脸量表 (RGS) 评估术后急性疼痛。在另一项实验中，使用α2-肾上腺素受体拮抗剂阿替美唑测试了α2-肾上腺素受体的参与情况。结果在假手术动物中，与所有组中相应的基线值相比，假手术后2小时的RGS没有增加。然而，在手术大鼠中，LPS组的术后RGS值显着高于对照组，表明LPS诱导的术后痛觉过敏。术中给予 DEX 可以预防 LPS 引起的切口后疼痛加剧。此外，术中DEX的预防作用受到阿替美唑预处理的抑制。结论我们的研究结果表明，术中DEX治疗可以通过α2-肾上腺素受体信号通路预防LPS引起的切口后疼痛加剧。
PurposeLow-grade endotoxin (lipopolysaccharide; LPS) exposure may contribute to the development of exaggerated acute postoperative pain. In the present study, we investigated the possible impact of intraoperative administration of dexmedetomidine (DEX) on LPS-induced postoperative hyperalgesia in a rat incisional pain model.MethodsThe surgical and sham-surgical animals were randomly divided into saline-treated control, 5.0 mg/kg LPS-treated, 10 µg/kg DEX-treated, and 5.0 mg/kg LPS + 10 µg/kg DEX-treated groups. In the surgical animals, a 1-cm-long plantar incision was made through the skin and fascia under isoflurane anesthesia. The sham-surgical rats were only anesthetized. All treatments were administered by a single intraperitoneal (i.p.) injection 60 min before surgery. Acute postoperative pain was assessed using the Rat Grimace Scale (RGS) one day before surgery (baseline) and at 2 h post incision. In another experiment, the involvement of the α2-adrenergic receptor was tested using atipamezole, an α2-adrenergic receptor antagonist.ResultsIn the sham-surgical animals, the RGS did not increase at 2 h after sham surgery compared with the corresponding baseline values in all groups. In the surgical rats, however, the postoperative RGS value of the LPS group was significantly higher than the control group, indicating LPS-induced postoperative hyperalgesia. Administration of intraoperative DEX could prevent the development of such LPS-induced exacerbated post-incisional pain. In addition, the preventive effects of intraoperative DEX were inhibited by pretreatment with atipamezole.ConclusionOur findings indicate that intraoperative DEX treatment can prevent LPS-induced exacerbated post-incisional pain via the α2-adrenergic receptor signaling pathway.