Electrochemical immunosensors for the simultaneous detection of two tumor markers
Electrochemical immunosensors for the simultaneous detection of two tumor markers
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DOI：
10.1021/ac0485278
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发表时间：
2005-03-01
影响因子：
7.4
通讯作者：
Wilson, MS
中科院分区：
文献类型：
--
作者：
Wilson, MS
描述了用于同时检测两种蛋白质分析物的电化学免疫传感器的微加工。传感器由两个直径为1毫米的氧化铱电极组成，电极在玻璃基板上形成图案。采用（3-氨基丙基）三乙氧基硅烷和戊二醛共价连接的方法将捕获的抗体固定在多孔氧化铱电极上。电极的空间分离（2.5 mm）使电化学免疫分析同时进行，电极之间没有串扰。采用电化学酶联免疫吸附试验测定蛋白质，电化学氧化碱性磷酸酶生成对苯二酚检测。同时检测山羊IgG和小鼠IgG以及肿瘤标志物CEA和AFP的传感器已研制成功。该传感器对山羊IgG、小鼠IgG、CEA和AFP的检出限分别为1、2、1.2和1 ng/mL。
The microfabrication of electrochemical immunosensors for the simultaneous detection of two protein analytes is described. The sensors consisted of two iridium oxide electrodes (1-mm diameter) patterned on a glass substrate. Capture antibodies were immobilized on the porous iridium oxide electrodes by covalent attachment using (3-aminopropyl)triethoxysilane and glutaraldehyde. The spatial separation of the electrodes (2.5 mm) enabled simultaneous electrochemical immunoassays to be conducted without cross-talk between the electrodes. Proteins were measured using electrochemical ELISA, and detection was achieved by electrochemically oxidizing alkaline phosphatase-generated hydroquinone. Sensors for the simultaneous detection of goat IgG and mouse IgG, and for the tumor markers CEA and AFP, were developed. The sensors had detection limits of 1, 2, 1.2, and I ng/mL for goat IgG, mouse IgG, CEA, and AFP, respectively.