Increased expression of Annexin A2 in oral squamous cell carcinoma
Increased expression of Annexin A2 in oral squamous cell carcinoma
复制标题
口腔鳞状细胞癌中膜联蛋白 A2 的表达增加。
DOI：
10.1016/j.archoralbio.2008.08.006
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发表时间：
2009-01-01
影响因子：
3
通讯作者：
Zhang, Zhi-yuan
中科院分区：
文献类型：
--
作者：
Zhong, Lai-ping;Wei, Kui-jie;Zhang, Zhi-yuan
以往，口腔鳞状细胞癌(OSCC)的体外细胞癌变模型是由一株人永生化口腔上皮细胞(HIOECs)、一株癌变的HB96细胞和另一种处于癌变早期的细胞(HB56细胞)建立的。在这项研究中，比较蛋白质组学分析确定了一组在这些细胞中差异表达的蛋白质，Annexin A2显示为伴随细胞转化而显著上调的蛋白质之一。进一步验证了Annexin A2在这三种细胞中的表达，以及在口腔鳞癌患者的临床标本和癌旁正常上皮中的表达。Western印迹分析和实时定量聚合酶链式反应检测到HIOECs上癌HB56和HB96细胞Annexin A2蛋白和mRNA水平升高。免疫组织化学结果显示，口腔鳞癌患者癌组织中Annexin A2蛋白的表达高于癌旁非癌组织，而癌组织与正常组织之间的表达水平无明显变化。有趣的是，Annexin A2蛋白表达水平与肿瘤分化程度呈负相关。提示Annexin A2蛋白可能在口腔鳞癌的发生发展中起重要作用，也可作为口腔鳞癌分化程度的候选生物标志物。(C)2008爱思唯尔有限公司。保留所有权利。
Previously, in vitro cellular carcinogenesis model of oral squamous cell carcinoma (OSCC) was established with a line of human immortalized oral epithelia cells (HIOECs), a line of cancerous HB96 cells, and another kind of cells (HB56 cells) at the early stage of carcinogenesis. In this study, comparative proteomic analysis identified a panel of differentially expressed proteins among these cells, and Annexin A2 shown as one of the significantly up-regulated proteins accompanying cellular transformation. Annexin A2 was further validated for its expression in the three kinds of cells and in the clinical samples of tumour tissues and their adjacent normal epithelia from primary OSCC patients. Western blot analysis and real-time PCR detected increased Annexin A2 protein and mRNA levels in cancerous HB56 and HB96 cells over HIOECs. Immunohistochemistry showed elevated Annexin A2 protein expression in tumour tissues over the adjacent non-malignant epithelia from OSCC patients; however, the mRNA levels between tumour and normal tissues did not change significantly. Interestingly, levels of Annexin A2 protein expression negatively correlated with the tumour differentiation grades. The results presented here suggest that Annexin A2 protein may play important roles in carcinogenesis of OSCC, and it may also serve as a candidate biomarker for pathologic differentiation grade of OSCC. (C) 2008 Elsevier Ltd. All rights reserved.