The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma
The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma
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DOI：
10.1093/intimm/dxm041
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发表时间：
2007-06-01
影响因子：
4.4
通讯作者：
Amagai, Takashi
中科院分区：
文献类型：
--
作者：
Mori, Kazuya;Itoi, Manami;Amagai, Takashi
已知淋巴样细胞的选定群体通过皮质-髓质交界处和髓质中的血管迁移入和迁移出成人胸腺。在这里，我们表明，在周围的大血管，CD 117阳性造血祖细胞，CD 4单阳性（SP）和CD 8 SP T细胞的小鼠血管周围空间（PVS）的位置。然而，发育中的胸腺细胞，CD 25阳性细胞和CD 4和CD 8双阳性细胞，在PVS中检测不到。静脉内（i. v.）将来自C57 BL/6小鼠的CD 117阳性骨髓（BM）细胞注射到未辐照的RAG 2突变小鼠Lv.中，供体来源的细胞首先在30分钟内优先迁移到PVS中，然后胸腺实质中的供体来源的细胞的数量增加。同样，在RAG 2突变小鼠胸腺实质中新发育的成熟T细胞转移到野生型BM细胞迁移到PVS，然后离开胸腺进入循环。用1-磷酸鞘氨醇受体激动剂FTY 720处理后，不仅在髓质而且在胸腺PVS中观察到成熟T细胞的积累。这些结果表明，PVS是一个过境途径祖细胞迁移到胸腺和成熟T细胞从胸腺迁出。
It is known that selected populations of lymphoid cells migrate into and from the adult thymus through blood vessels at the cortico-medullary junction and in the medulla. Here, we show that in the perivascular spaces (PVS) of mice surrounding large blood vessels, CD117-positive hematopoietic progenitor cells, CD4 single-positive (SP) and CD8SP T cells are located. However, developing thymocytes, CD25-positive cells and CD4 and CD8 double-positive cells, are not detectable in the PVS. After intravenous (i.v.) injection of CD117-positive bone marrow (BM) cells from C57BL/6 mice into non-irradiated RAG2 mutant mice Lv., donor-derived cells first preferentially migrate into the PVS within 30 min, and then the number of donor-derived cells in the thymic parenchyma increases. Likewise, newly developed mature T cells in the thymic parenchyma of RAG2 mutant mice transferred with wild-type BM cells migrate to the PVS, before leaving the thymus to the circulation. Accumulation of mature T cells was observed after treatment with sphingosine-1 phosphate receptor agonist FTY720 not only in the medulla but also in the thymic PVS. These results suggest that the PVS is a transit pathway for progenitor cells to immigrate into the thymus and for mature T cells to emigrate from the thymus.