Comparative analysis of the LPS biosynthetic loci of the genetic subtypes of serovar Hardjo:: Leptospira interrogans subtype Hardjoprajitno and Leptospira borgpetersenii subtype Hardjobovis
Comparative analysis of the LPS biosynthetic loci of the genetic subtypes of serovar Hardjo:: Leptospira interrogans subtype Hardjoprajitno and Leptospira borgpetersenii subtype Hardjobovis
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DOI：
10.1111/j.1574-6968.1999.tb13749.x
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发表时间：
1999-08-15
影响因子：
2.1
通讯作者：
Adler, B
中科院分区：
文献类型：
--
作者：
de la Peña-Moctezuma, A;Bulach, DM;Adler, B
虽然伯氏钩端螺旋体（Leptospiraborgpetersenii）的Hardjobovis亚型和L.问号线虫亚型Hardjoprajitno属于不同的物种，它们在血清学上是不可区分的，因此被分类为血清型Hardjo。由于LPS是参与血清学分类的主要抗原，这意味着这些亚型的LPS是相同的。LPS生物合成位点（RFB）的亚型的比较显示显着的相似性，与32和31个复制起点（ORFs）在Hardjoprajitno和Hardjobovis RFB位点，分别。这些ORF的顺序和方向是相同的，除了在Hardjoprajitno的ORF 4和5之间的一个额外的ORF和亚型之间的基因间序列不同。Hardjoprajitno rfb基因座已被分为四个插入区域的基础上，其他钩端螺旋体rfb基因座的序列相似性。orfJ 1-orfJ 14和orfJ 21-orfJ 22与L.哈氏牛亚种orfJ 15-orfJ 20和orfJ 23-orfJ 31与L.哥本哈根问号线虫我们提出，祖先Hardjoprajitno菌株，包含一个rfb基因座，非常相似的Copenhageni基因座，收购ORFs 1-14和ORFs 21-22从亚型Hardjobovis导致两个血清学上难以区分的亚型的血清型Hardjo，这反过来又构成了主要的牛适应钩端螺旋体血清型。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Although Leptospira borgpetersenii subtype Hardjobovis and L. interrogans subtype Hardjoprajitno belong to different species, they are serologically indistinguishable and are therefore classified as serovar Hardjo. Since LPS is the major antigen involved in serological classification, this implies that the LPS of these subtypes is identical. Comparison of the LPS biosynthetic loci (rfb) of the subtypes revealed remarkable similarity, with 32 and 31 origins of replication (orfs) in the Hardjoprajitno and Hardjobovis rfb loci, respectively. The order and orientation of these orfs were identical with the exception of an additional orf in Hardjoprajitno between orfs 4 and 5 and intergenic sequences differing between the subtypes. The Hardjoprajitno rfb locus has been divided into four intercalated regions based on sequence similarity to other leptospiral rfb loci. orfJ1-orfJ14 as well as orfJ21-orfJ22 are more similar to regions of the rfb locus of L. borgpetersenii subtype Hardjobovis. orfJ15-orfJ20 as well as orfJ23-orfJ31 are almost identical to the corresponding orfs in L. interrogans serovar Copenhageni. We propose that the progenitor Hardjoprajitno strain, containing an rfb locus which closely resembled the Copenhageni locus, acquired orfs 1-14 and orfs 21-22 from subtype Hardjobovis resulting in two serologically indistinguishable subtypes of serovar Hardjo which in turn constituted the main bovine-adapted leptospiral serovar. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.