1-bromopropane, an alternative to ozone layer depleting solvents, is dose-dependently neurotoxic to rats in long-term inhalation exposure
1-bromopropane, an alternative to ozone layer depleting solvents, is dose-dependently neurotoxic to rats in long-term inhalation exposure
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DOI：
10.1093/toxsci/55.1.116
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发表时间：
2000-05-01
影响因子：
3.8
通讯作者：
Takeuchi, Y
中科院分区：
文献类型：
--
作者：
Ichihara, G;Kitoh, J;Takeuchi, Y
1-溴丙烷是新推出的，作为消耗臭氧层溶剂的替代品。我们的目的是阐明 1-溴丙烷对神经系统的剂量依赖性影响。 44只Wistar雄性大鼠随机分为4组，每组11只。这些组每天接触 200、400 或 800 ppm 1-溴丙烷或仅接触新鲜空气 8 小时，持续 12 周。每4周测定每组9只大鼠的前肢和后肢握力、最大运动神经传导速度（MCV）和尾神经远端潜伏期（DL）。每组另2只大鼠于实验结束时进行灌注，进行形态学检查。 800 ppm 组的大鼠踢腿能力较差，无法在斜坡上保持静止。暴露 12 周后，前肢握力在 800 ppm 时显着下降，后肢握力在 400 和 800 ppm 时或暴露 12 周后显着下降。尾神经的 MCV 和 DL 在 800 ppm 时显着恶化。在 800 ppm 组中，在未解开的胫后神经肌支中，髓鞘的卵形或气泡状碎片很明显。薄核中前终末轴突的肿胀以剂量依赖性方式增加。血浆肌酸磷酸激酶 (CPK) 呈剂量依赖性下降，在 400 和 800 ppm 时变化显着。 1-溴丙烷以剂量依赖性方式引起大鼠四肢肌力减弱、MCV 和 DL 恶化，并引起周围神经和薄核中前终末轴突的形态变化。 1-溴丙烷可能对人类有严重的神经毒性，因此在工作场所应谨慎使用。
1-Bromopropane has been newly introduced as an alternative to ozone layer-depleting solvents. We aimed to clarify the dose-dependent effects of 1-bromopropane on the nervous system. Forty-four Wistar male rats were randomly divided into 4 groups of 11 each. The groups were exposed to 200, 400, or 800 ppm of 1-bromopropane or only fresh air 8 h per day for 12 weeks. Grip strength of forelimbs and hind limbs, maximum motor nerve conduction velocity (MCV), and distal latency (DL) of the tail nerve were measured in 9 rats of each group every 4 weeks. The other 2 rats of each group were perfused at the end of the experiment for morphological examinations. The rats of the 800-ppm group showed poor kicking and were not able to stand still on the slope. After a 12-week exposure, forelimb grip strength decreased significantly at 800 ppm and hind limb grip strength decreased significantly at both 400 and 800 ppm or after a 12-week exposure. MCV and DL of the tail nerve deteriorated significantly at 800 ppm. Ovoid or bubblelike debris of myelin sheaths was prominent in the unraveled muscular branch of the posterior tibial nerve in the 800-ppm group. Swelling of preterminal axons in the gracile nucleus increased in a dose-dependent manner. Plasma creatine phosphokinase (CPK) decreased dose-dependently with significant changes at 400 and 800 ppm. 1-Bromopropane induced weakness in the muscle strength of rat limbs and deterioration of MCV and DL in a dose-dependent manner, with morphological changes in peripheral nerve and preterminal axon in the gracile nucleus. 1-Bromopropane may be seriously neurotoxic to humans and should thus be used carefully in the workplace.