Permeation of blood-borne IL15 across the blood-brain barrier and the effect of LPS
Permeation of blood-borne IL15 across the blood-brain barrier and the effect of LPS
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DOI：
10.1111/j.1471-4159.2008.05390.x
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发表时间：
2008-07-01
影响因子：
4.7
通讯作者：
Kastin, A. J.
中科院分区：
文献类型：
--
作者：
Pan, W.;Hsuchou, H.;Kastin, A. J.
白细胞介素15（IL 15）是一种促炎细胞因子，在涉及外周（例如类风湿性关节炎）和CNS（例如多发性硬化）的自身免疫性疾病中浓度升高。在正常和脂多糖（LPS）处理的小鼠中研究了其与血脑屏障（BBB）的相互作用。I-125-IL 15在静脉注射后至少10分钟内保持完整，并到达CNS实质，脑和脊髓之间存在区域差异。在体和原位脑灌注实验均表明，I-125-IL 15对血脑屏障的渗透是不饱和的。LPS诱导脑和脊髓的IL 15摄取显著增加，部分与BBB的更高的一般渗透性有关。结果表明，血脑屏障是一个界面，血液传播的IL 15与中枢神经系统在基础状态和炎症过程中相互作用。
Interleukin15 (IL 15) is a proinflammatory cytokine with elevated concentrations in autoimmune diseases involving the periphery (e.g. rheumatoid arthritis) and CNS (e.g. multiple sclerosis). Its interactions with the blood-brain barrier (BBB) were studied in normal and lipopolysaccharide (LPS)-treated mice. I-125-IL15 remained intact for at least 10 min after i.v. injection and reached CNS parenchyma with regional differences between brain and spinal cord. Both in vivo and in situ brain perfusion of I-125-IL15 showed that its permeation of the BBB was non-saturable. LPS induced a significant increase of IL15 uptake by the brain and spinal cord, partly related to a higher general permeability of the BBB. The results suggest that the BBB is an interface for blood-borne IL15 to interact with the CNS in the basal state and during inflammation.