COXSACKIEVIRUS B3 INFECTION ALTERS THE UPDATE OF 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN INTO VARIOUS TISSUES OF THE MOUSE
COXSACKIEVIRUS B3 INFECTION ALTERS THE UPDATE OF 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN INTO VARIOUS TISSUES OF THE MOUSE
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DOI：
10.1016/0300-483x(94)90202-x
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发表时间：
1994-05-31
期刊：
影响因子：
4.5
通讯作者：
ILBACK, NG
中科院分区：
文献类型：
--
作者：
FUNSETH, E;ILBACK, NG
本文研究了2，3，7，8-四氯二苯并-对二恶英(TCDD)在小鼠模型中的组织摄取。雄性A/J小鼠感染CB3病毒后，在感染后第4天和第7天分别注射0.5mCi[C-14]TCDD(约65mU g/kg)。未感染组注射[C-14]TCDD作为对照组。注射同位素后24 h取脾、胸腺、心脏、胰腺、肝脏和脑组织，用标准液体闪烁技术测定组织中[C-14]TCDD含量。第7天，注射同位素后4h行全身放射自显影。我们的结果清楚地表明，在CB3感染期间，选定的组织对TCDD的摄取增加，这种摄取增加在接种后第4天最为明显，这对应于病毒血症的峰值。结果发现，TCDD在脑、胰腺、心脏、脾和肝脏的总组织浓度在第4天显著升高。在脾、心和脑中，它们在第7天仍显著增加。通常由感染引起的相当大的代谢和免疫变化可能解释了TCDD积聚的变化。TCDD摄取的增加可能会影响参与炎性组织损伤修复的免疫细胞，从而影响疾病的进展。
The tissue uptake of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) during infection was studied in a mouse model using the common human virus Coxsackievirvs B3 (CB3), adapted here to the mouse. Male A/J-mice were infected with the CB3 virus and subsequently, on days 4 and 7 of the infection, injected with 0.5 mu Ci [C-14]TCDD (about 65 mu g/kg). An uninfected group was injected with [C-14]TCDD and served as the control group. Spleen, thymus, heart, pancreas, liver and brain were dissected 24 h after isotope injection, and the [C-14]TCDD content of the tissues was determined by standard liquid scintillation techniques. On day 7, whole body autoradiography was also performed, 4 h after the isotope injection. Our results clearly show that there is an increased TCDD uptake in the selected tissues during the CB3 infection and that this increased uptake is most pronounced on day 4 post inoculation, which corresponds to the peak of viraemia. It was found that the total tissue concentrations of TCDD were significantly increased on day 4 in the brain, pancreas, heart, spleen and liver. In the spleen, heart and brain they were still significantly increased on day 7. The considerable metabolic and immunological changes that generally are caused by infections might explain the change in accumulation of TCDD. It is possible that the increased uptake of TCDD affects the immune cells involved in repair of inflammatory tissue lesions, thereby affecting the progression of the disease.