Measurement of urinary zearalenone concentrations for monitoring natural feed contamination in cattle herds: On-farm trials
Measurement of urinary zearalenone concentrations for monitoring natural feed contamination in cattle herds: On-farm trials
复制标题
DOI：
10.2527/jas.2010-3306
复制
发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Fink-Gremmels, J.
中科院分区：
文献类型：
--
作者：
Takagi, M.;Uno, S.;Fink-Gremmels, J.
本研究的目的是研究使用市售的 ELISA 方法在不同饲养条件下饲养的牛中测量牛尿玉米赤霉烯酮 (ZEN) 浓度的效果，以监测农场饲料的自然污染，并根据尿 ZEN 浓度研究在饲料中添加霉菌毒素吸附剂 (MA) 产品的效果。首先，为繁殖（4 群）和育肥（4 群）目的而饲养的日本黑牛群具有类似的饲养条件。然后，收集并分析每群 5 头奶牛的尿液样本。其次，选择 1 群饲喂总混合日粮 (TMR) 的奶牛。将MA（40克/天）与TMR充分混合后，按照以下时间表饲喂补充的TMR：添加MA 2周，不添加MA 3周；然后使用 MA 2 周，不使用 MA 6 周。从奶牛（n = 6 至 7）收集尿液样本，并在每个间隔之前和之后进行检查。通过ELISA和液相色谱-串联质谱法测量玉米赤霉烯酮浓度。 ZEN及其代谢物的浓度以肌酐(Crea)校正后表示[ZEN或代谢物(pg/mL)/Crea(mg/dL)； pg/mg Crea]。在第一个实验中，ZEN 及其代谢物的尿液浓度在所有牛群中都有所不同，并且在牛群之间观察到显着差异。特别是在 1 个育肥牛群中，尿 ZEN 浓度高于其他 3 个牛群（P < 0.001）。这可能反映了农场饲料中存在严重的自然 ZEN 污染。在Exp。 2、补充MA后尿ZEN浓度表现出特殊的趋势。补充 2 周后，观察到 ZEN 显着下降（P < 0.05）。在不补充 MA 的情况下 3 周和补充 MA 的 2 周期间，玉米赤霉烯酮浓度保持在较低的水平。当接下来的 6 周内不再向饲料中添加 MA 时，浓度会增加至原始数量。这些发现表明，测量尿液中 ZEN 及其代谢物的浓度不仅可以用于监测农场水平牛饲料的天然 ZEN 污染，还可以用于在饲料中添加 MA 后对 MA 功能进行体内评估。
The aims of the present study were to investigate the efficacy of measuring bovine urinary zearalenone (ZEN) concentrations by using a commercially available ELISA method in cattle kept under different feeding conditions to monitor the natural contamination of feeds at the farm level, and to investigate the effects of supplementation of a mycotoxin adsorbent (MA) product in the feed based on urinary ZEN concentration. First, Japanese Black cattle herds kept for breeding (4 herds) and fattening (4 herds) purposes were provided with similar feeding conditions. Then, urinary samples from 5 cows in each herd were collected and analyzed. Second, dairy cows from 1 herd fed with total mixed rations (TMR) were selected. After thorough mixing of the MA (40 g/d) with TMR, the supplemented TMR was fed according to the following schedule: with MA for 2 wk, without MA for 3 wk; then with MA for 2 wk and without MA for 6 wk. Urine samples were collected from cows (n = 6 to 7) and examined before and after each interval. Zearalenone concentrations were measured by the ELISA and liquid chromatography-tandem mass spectrometry methods. The concentration of ZEN and its metabolites was expressed after creatinine (Crea) correction [ ZEN or metabolites (pg/mL)/Crea (mg/dL); pg/mg of Crea]. In the first experiment, the urinary concentrations of ZEN and its metabolites were variable in all herds, and significant differences were observed between herds. In 1 fattening herd, in particular, urinary ZEN concentrations were greater (P < 0.001) than in the other 3 herds. This might reflect significant natural ZEN contamination of the feed at the farm level. In Exp. 2, urinary ZEN concentrations displayed peculiar trends after supplementation with MA. After 2 wk of supplementation, a significant decrease of ZEN (P < 0.05) was observed. Zearalenone concentrations remained at a reduced amount during 3 wk without MA supplementation and 2 wk with MA supplementation. When MA was not added to the feed for the next 6 wk, the concentrations increased to the original quantity. These findings indicate the usefulness of measuring concentrations of urinary ZEN and its metabolites not only for monitoring the natural ZEN contamination of cattle feed at the farm level but also for in vivo evaluation of MA function after supplementing feeds with MA.