Contribution to the physically anchored linkage map of the pig.
Contribution to the physically anchored linkage map of the pig.
复制标题
对猪的物理锚定连锁图的贡献。
DOI：
10.1111/j.1365-2052.1997.00084.x
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发表时间：
1997
期刊：
影响因子：
2.4
通讯作者：
J. Gellin
中科院分区：
文献类型：
--
作者：
A. Robic;D. Milan;N. Woloszyn;J. Riquet;M. Yerle;M. Nagel;M. Bonnet;P. Pinton;M. Dalens;J. Gellin
已在PiGMaP猪遗传图谱上定位了33个微卫星。与已发表的PiGMaP图谱相比，扩展了2号染色体(140 cM/70 cM)和3号染色体(180 cM/110 cM)的图谱，并在7号染色体的p臂末端和15号染色体的着丝粒末端定位了新的标记。对3号和17号染色体的标记提出了新的顺序。我们测试了亚克隆连接混合聚合酶链式反应(SLIM-PCR)从宇宙中分离微卫星的方法。当粘粒含有多个微卫星时，亚克隆更有效，而当粘粒包含单个微卫星时，SLIM-PCR更直接。使用混合细胞板对15个匿名微卫星进行了地区性分配。对于地图整合，通过使用杂交细胞小组确定匿名微卫星的区域分配提供了一种替代方案，即通过原位杂交将微卫星从宇宙中分离出来并进行定位。
Thirty-three microsatellites have been mapped on the PiGMaP porcine genetic map. By comparison with the previously published PiGMaP maps, the maps of chromosome 2 (140 cM/70 cM) and chromosome 3 (180 cM/110 cM) were extended and new markers were mapped on the p-arm extremity of chromosome 7 and on the centromeric extremity of chromosome 15. New orders are proposed for markers on chromosomes 3 and 17. Six microsatellites isolated from cosmids were also localized on the cytogenetic map by fluorescent in situ hybridization. We tested the subcloning ligation mixture-polymerase chain reaction (SLiM-PCR) method for isolating microsatellites from cosmids. Subcloning is more effective when the cosmid harbours several microsatellites whereas SLiM-PCR is more straightforward when the cosmid contains a single microsatellite. Fifteen anonymous microsatellites were regionally assigned by using a hybrid cell panel. For map integration, the determination of a regional assignment of anonymous microsatellites by using a hybrid cell panel offers an alternative to microsatellite isolation from cosmids and their localizations by in situ hybridization.