PREDICTION OF PROPERTIES OF FRACTURE CALLUS BY MEASUREMENT OF MINERAL DENSITY USING MICRO-BONE DENSITOMETRY
PREDICTION OF PROPERTIES OF FRACTURE CALLUS BY MEASUREMENT OF MINERAL DENSITY USING MICRO-BONE DENSITOMETRY
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DOI：
10.2106/00004623-198971070-00008
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发表时间：
1989-08-01
影响因子：
5.3
通讯作者：
CHAO, EYS
中科院分区：
文献类型：
--
作者：
ARO, HT;WIPPERMANN, BW;CHAO, EYS
设计了一项实验来确定是否可以通过测量矿物密度来预测骨折愈伤组织的材料特性。使用具有闭合性骨折的大鼠胫骨在连续愈合阶段获得均匀的骨折愈伤组织标本。进行体外压痕测试，并使用环钻对组织进行活检，以确定愈伤组织标准化区域的硬度和钙含量。愈伤组织的硬度与局部组织的矿物质密度相关，该密度最初是通过基于单光子的微骨密度测定法进行非侵入性测量的。通过组织的比较平面分析和显微放射照相分析来确定愈伤组织的分化和重塑进程。密度测定显示，与羟基磷灰石当量溶液的浓度（r = 0.997，p < 0.001）、皮质小梁骨对照样本的灰分重量（r = 0.998，p < 0.001）以及骨折愈伤组织的钙含量（微克/立方毫米）（r = 0.854，p < 0.001）呈线性相关。通过微骨密度测定法测量，愈伤组织的硬度与单位体积组织的矿物质密度呈非线性关系（r = 0.959，p < 0.005）。传统X光片上骨折骨痂的出现是评估骨折愈合的重要临床发现。然而，它并不能可靠地预测骨折愈合的强度。骨矿物质的三维成像和定量提供了有关骨折骨痂质量和几何形状的附加数据。我们的研究表明，单位体积的矿物质含量是骨折愈伤组织硬度的良好指标。因此，定量成像在临床上可用于监测骨折愈合。
An experiment was designed to determine whether the material properties of fracture callus can be predicted by the measurement of mineral density. Rat tibiae that had a closed fracture were used to obtain uniform specimens of fracture callus at sequential stages of healing. An in vitro indentation test was done and tissue was biopsied, using a trephine, to determine the hardness and calcium content of a standardized area of callus tissue. The hardness of the callus was correlated with the mineral density of the local tissue, which initially had been measured non-invasively with single-photon based micro-bone densitometry. The progress of differentiation and remolding of callus was determined by comparative planimetric and microradiographic analyses of tissue. Determinations of density showed a linear correlation with concentrations of a hydroxyapatite equivalent solution (r = 0.997, p < 0.001), with the ash weight of control specimens of cortical-trabecular bone (r = 0.998, p < 0.001), and with the calcium content (micrograms per cubic millimeter) of the fracture callus (r = 0.854, p < 0.001). The hardness of the callus had a non-linear relationship (r = 0.959, p < 0.005) with the mineral density per volume of tissue, as measured by micro-bone densitometry. The appearance of fracture callus on conventional radiographs is an important clinical finding in the assessment of fracture-healing. It is, however, an unreliable predictor of the strength of the union of the fracture. Three-dimensional imaging and quantification of bone mineral provide additional data about the quality and geometry of fracture callus. Our study showed that mineral content per unit of volume is a good index of hardness of the fracture callus. Thus, quantitative imaging may be useful clinically for monitoring fracture-healing.