Two-dimensional 1H NMR studies of histidine-containing protein from Escherichia coli. 2. Leucine resonance assignments by long-range coherence transfer.
Two-dimensional 1H NMR studies of histidine-containing protein from Escherichia coli. 2. Leucine resonance assignments by long-range coherence transfer.
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来自大肠杆菌的含组氨酸蛋白质的二维 1H NMR 研究。
DOI：
10.1021/bi00371a072
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Drobny,GP
中科院分区：
文献类型：
--
作者：
Klevit,RE;Drobny,GP
材料和方法核磁共振波谱仪。所有NMR实验均在自制的500-MHzNMR光谱仪上进行。射频部分采用双通道设计，每个通道基于PTS-500合成器; 500 MHz脉冲辐射由440 MHz本地振荡器和60 MHz中频混频产生。中频是从PTS-500频率合成器的10 MHz基准频率中获得的。每个60 MHz通道分为四个并行通道，其相位为正交。每个并行通道由一个射频开关和连续的相位和幅度调整。500 MHz功率放大器为ENI-525 LA，输出功率略高于25 W。500-MHz质子探针和宽带（75-500-MHz）锁定通道购自Cryomagnet Systems。控制台还配备了一个小角度射频移相器。该数据系统是基于一个微型VAX II计算机配备了一个350兆字节的密封磁盘和联网的第二个微型VAX II和VAX 1 1/780。脉冲编程器和数字采集处理器（DAP）是在华盛顿大学设计和制造的。脉冲编程器是一个80位宽，4096深随机存取存储器，配备了64个可嵌套的硬件循环。每个循环可以计数到216。在80位脉冲编程器字中，32位专用于定时，32位控制各种控制台功能。
Materials and Methods NMR Spectrometer. All NMR experiments were carried out on a home-built 500-MHzNMR spectrometer. The ra-dio-frequency (rf) section is dual-channel design, with each channel based on a PTS-500 synthesizer; 500-MHz pulsed irradiations are produced by mixing a 440-MHz local oscillator with a 60-MHz intermediatefrequency. The intermediate frequency is derived from the 10-MHz reference from the PTS-500 synthesizer. Each 60-MHz channel is split into four parallel channels, the phases of which are in quadrature. Each parallel channel consists of an rf switch and continuous phase and amplitude adjusts. The 500-MHz power amplifier is an ENI-525LA, which outputs just over 25 W. The 500-MHz proton probe and broad-band (75-500-MHz) lock channel were purchased from Cryomagnet Systems. The console is also equipped with a small-angle rf phase shifter. The data system is based on a micro-VAX II computer equipped with a 350-Mbyte sealed disk and networked to a second micro-VAX II and VAX 1 1/780. The pulse pro-grammer and digital acquisition processor (DAP) were de-signed and constructed at the University of Washington. The pulse programmer is an 80 bit wide by 4096 deep random access memory equipped with 64 nestable hardware loops. Each loop may count to 216. Within a 80-bit pulse program-mer word, 32 bits are dedicated to timing, and 32 bits control various console functions.