COMPARISON OF NEUROVIRULENCE OF DIFFERENT STRAINS OF YELLOW-FEVER VIRUS IN MICE
COMPARISON OF NEUROVIRULENCE OF DIFFERENT STRAINS OF YELLOW-FEVER VIRUS IN MICE
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DOI：
10.1099/0022-1317-67-4-631
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发表时间：
1986-04-01
影响因子：
3.8
通讯作者：
GOULD, EA
中科院分区：
文献类型：
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作者：
BARRETT, ADT;GOULD, EA
采用脑内和鼻内给药途径，比较了不同疫苗和野生型黄热病（YF）病毒对幼龄成年小鼠的毒力。35种不同的YF病毒在脑内接种后12天内杀死小鼠。相比之下，鼻内给药后，仅7只小鼠死亡;这些是Asibi病毒（YF-AS），法国嗜神经性疫苗，三种17 DD疫苗亚株病毒中的两种（巴西，哥伦比亚，但不是达喀尔）和六种野生型分离株中的三种（YF-B7，YF-B12和YF-B15）。来自17 D-204疫苗亚株的8种不同制备液经鼻内途径均无毒力。因此，如果使用鼻内途径，则可以基于它们对小鼠的毒力来区分YF病毒株。使用从野生型病毒储备中选择的大小噬斑获得了种群内小鼠毒力异质性的证据。鼻内接种后，YF-AS小空斑变异比其亲本病毒或大空斑变异更具毒性。另一方面，在这些试验中无法区分来自无毒力野生型菌株的大噬菌斑变体和小噬菌斑变体。
The virulence of different vaccine and wild-type yellow fever (YF) viruses for young adult mice was compared using both intracerebral and intranasal routes of administration. Thirty-five different YF viruses killed mice within 12 days following intracerebral inoculation. In contrast, only seven of those examined killed mice following intranasal administration; these were Asibi virus (YF-AS), the French neurotropic vaccine, two out of three 17DD vaccine substrain viruses (Brazil, Colombia but not Dakar) and three out of six wild-type isolates (YF-B7, YF-B12 and YF-B15). None of eight distinct preparations from the 17D-204 vaccine substrain was virulent by the intranasal route. Thus, strains of YF virus can be distinguished on the basis of their virulence for mice if the intranasal route is used. Evidence of heterogeneity in mouse virulence within the populations was obtained using large and small plaques selected from wild-type stocks of virus. Following intranasal inoculation, a YF-AS small plaque variant was more virulent than either its parent virus or a large plaque variant. On the other hand, a large and a small plaque variant from a non-virulent wild-type strain could not be distinguished in these tests.