INVIVO EFFECTS OF INTERLEUKIN-2 ON LYMPHOCYTE SUBPOPULATIONS IN A PATIENT WITH A COMBINED IMMUNODEFICIENCY
INVIVO EFFECTS OF INTERLEUKIN-2 ON LYMPHOCYTE SUBPOPULATIONS IN A PATIENT WITH A COMBINED IMMUNODEFICIENCY
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DOI：
10.1016/s0171-2985(84)80077-7
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发表时间：
1984-01-01
期刊：
影响因子：
2.8
通讯作者：
MUHLRADT, PF
中科院分区：
文献类型：
--
作者：
DOPFER, R;NIETHAMMER, D;MUHLRADT, PF
本报告描述了一项使用白细胞介素2 （IL-2）治疗17个月新生儿的临床试验。合并免疫缺陷（Nezelof综合征）的老年男童。白细胞介素-2是由植物血凝素刺激的白皮肤的条件培养基制备的。IL-2的纯化涉及到Matrex Blue A sepharose层析和凝胶过滤层析。该制剂不含巨噬细胞毒性因子、巨噬细胞迁移抑制因子和集落刺激因子。它含有可忽略不计的干扰素- γ活性。IL-2活性调整至1600 U/ml，对应于。apprx。0.8 .mu。g均相IL-2/ml。患者接受了为期50天的治疗，总剂量为20,000 U IL-2， sc注射。IL-2耐受性良好。在3周内，治疗导致前3个月普遍存在的淋巴细胞增多症正常化。明显的嗜酸性粒细胞也有所改善，但未达到正常水平。最显著的效果是OKT4+/OKT8+比值的正常化，同时外周血中OKT3+细胞的相对增加。对E莲座形成、B细胞计数或血清Ig水平没有影响。与治疗前一样，自然杀伤细胞或抗体依赖细胞的细胞毒性活性仍然很高。在IL-2治疗期间，感染发作和抗生素治疗的需求较少。IL-2的一些作用是短暂的，例如，OKT4+和OKT3+细胞的计数在停止治疗几周后恢复到病理值。
This report describes a clinical trial with interleukin 2 (IL-2) on a 17-mo. old male child with combined immunodeficiency (Nezelof''s syndrome). IL-2 was prepared from conditioned media of phytohemagglutinin-stimulated leukocytes from buffy coats. The purification of IL-2 involved chromatography on Matrex Blue A sepharose and gel filtration chromatography. The preparation was free of macrophage cytotoxicity factor, macrophage migration inhibition factor and colony-stimulating factor. It contained negligible activity of interferon-.gamma.. IL-2 activity was adjusted to 1600 U/ml, which corresponds to .apprx. 0.8 .mu.g homogeneous IL-2/ml. The patient was treated over a 50-day period with a total dose of 20,000 U IL-2, which was injected s.c. IL-2 was well tolerated. Within 3 wk, the treatment led to a normalization of a lymphocytosis which had prevailed for the previous 3 mo. A pronounced eosinophila also improved but did not reach normal levels. The most striking effect was a normalization of the OKT4+/OKT8+ ratio with a concomitant relative increase in OKT3+ cells in the peripheral blood. No effects were seen on E rosette formation, B cell counts or serum Ig levels. Natural killer cell or antibody-dependent cellular cytotoxicity activity remained high, as before the treatment. Infectious episodes and requirement for antibiotic treatment were less frequent during IL-2 therapy. Some effects of IL-2 were transient, e.g., the counts of OKT4+ and OKT3+ cells, which returned to pathological values a few weeks after the treatment was discontinued.