Urinary tract colonization is enhanced by a plasmid that regulates uropathogenic Acinetobacter baumannii chromosomal genes
Urinary tract colonization is enhanced by a plasmid that regulates uropathogenic Acinetobacter baumannii chromosomal genes
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DOI：
10.1038/s41467-019-10706-y
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发表时间：
2019-06-24
影响因子：
16.6
通讯作者：
Feldman, Mario F.
中科院分区：
文献类型：
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作者：
Di Venanzio, Gisela;Flores-Mireles, Ana L.;Feldman, Mario F.
多重耐药鲍曼不动杆菌（MDR）对全球健康构成日益严重的威胁。关于不动杆菌致病机理的研究主要集中在肺炎和血液感染上，尽管五分之一的不动杆菌属细菌在感染过程中会引起肺炎和血液感染。鲍曼不动杆菌菌株分离自泌尿部位。在这项研究中，我们强调了A。鲍曼不动杆菌作为泌尿道病原体。我们开发了第一个A。鲍曼不动杆菌导管相关尿路感染（BUTI）小鼠模型，使用UPAB1，一种最近的MDR泌尿分离株。UPAB1携带质粒pAB5，其是抑制多种不动杆菌属菌株中的VI型分泌系统（T6SS）的大接合质粒家族的成员。pAB 5赋予生态位特异性，因为它的携带改善了UPAB1在肺炎模型中的存活并降低了肺炎模型中的毒力。比较蛋白质组学和转录组学分析表明，除了T6SS，pAB 5调节多个染色体编码的毒力因子的表达。我们的研究结果表明，质粒可以通过控制染色体基因的表达来影响细菌感染。
Multidrug resistant (MDR) Acinetobacter baumannii poses a growing threat to global health. Research on Acinetobacter pathogenesis has primarily focused on pneumonia and bloodstream infections, even though one in five A. baumannii strains are isolated from urinary sites. In this study, we highlight the role of A. baumannii as a uropathogen. We develop the first A. baumannii catheter-associated urinary tract infection (CAUTI) murine model using UPAB1, a recent MDR urinary isolate. UPAB1 carries the plasmid pAB5, a member of the family of large conjugative plasmids that represses the type VI secretion system (T6SS) in multiple Acinetobacter strains. pAB5 confers niche specificity, as its carriage improves UPAB1 survival in a CAUTI model and decreases virulence in a pneumonia model. Comparative proteomic and transcriptomic analyses show that pAB5 regulates the expression of multiple chromosomally-encoded virulence factors besides T6SS. Our results demonstrate that plasmids can impact bacterial infections by controlling the expression of chromosomal genes.