Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage.
Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage.
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DOI：
10.1021/acssensors.7b00861
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发表时间：
2018-02
期刊：
影响因子：
8.9
通讯作者：
Scarlet Milo;Florianne B. Acosta;H. Hathaway;Laura A Wallace;N. Thet;A. Jenkins
中科院分区：
文献类型：
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作者：
Scarlet Milo;Florianne B. Acosta;H. Hathaway;Laura A Wallace;N. Thet;A. Jenkins
变形杆菌感染后结晶生物膜的形成可导致长期留置导管的结痂和堵塞，具有严重的临床后果。我们描述了一种简单的传感器，放置在导尿管引流袋内，通过尿液颜色的变化来警告即将发生的阻塞。ph响应传感器是一种双层聚合物“含片”，能够释放自猝灭染料5(6)-羧基荧光素，以响应细菌脲酶表达产生的碱性尿液。在实验室尿路模型中评估传感器的性能，在确定感染的条件下感染脲酶阳性和阴性细菌菌株，平均达到14.5小时的导管阻塞“预警”。仅在脲酶阳性细菌感染后才会发生信号。将这些传感器转化到临床环境中，可以在导管堵塞发生之前进行适当的干预，这是目前没有有效控制方法的问题。
Formation of crystalline biofilms following infection by Proteus mirabilis can lead to encrustation and blockage of long-term indwelling catheters, with serious clinical consequences. We describe a simple sensor, placed within the catheter drainage bag, to alert of impending blockage via a urinary color change. The pH-responsive sensor is a dual-layered polymeric "lozenge", able to release the self-quenching dye 5(6)-carboxyfluorescein in response to the alkaline urine generated by the expression of bacterial urease. Sensor performance was evaluated within a laboratory model of the catheterized urinary tract, infected with both urease positive and negative bacterial strains under conditions of established infection, achieving an average "early warning" of catheter blockage of 14.5 h. Signaling only occurred following infection with urease positive bacteria. Translation of these sensors into a clinical environment would allow appropriate intervention before the occurrence of catheter blockage, a problem for which there is currently no effective control method.