In vivo enhancement of sensory perception recovery in a tissue-engineered skin enriched with laminin
In vivo enhancement of sensory perception recovery in a tissue-engineered skin enriched with laminin
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DOI：
10.1016/j.biomaterials.2006.01.014
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发表时间：
2006-05-01
期刊：
影响因子：
14
通讯作者：
Berthod, F
中科院分区：
文献类型：
--
作者：
Caissie, R;Gingras, M;Berthod, F
使用自体重建皮肤似乎是一个有前途的治疗永久覆盖深度和广泛的烧伤。然而，移植到伤口上的重建皮肤促进感觉知觉恢复的能力是一个主要问题。我们的目的是评估层粘连蛋白对皮肤神经再生的影响。我们制备了富含0、1、10或50 μ g层粘连蛋白/海绵的胶原-壳聚糖海绵，通过培养人成纤维细胞和角质形成细胞来产生组织工程化重建皮肤，然后移植到无胸腺小鼠背部120天。免疫组织化学研究表明，有7倍以上的神经丝150 kD阳性神经纤维迁移在移植物中的样品中富含10 μ g层粘连蛋白/海绵，相比，重建皮肤无层粘连蛋白，移植后120天。在所有富含层粘连蛋白的移植物中，使用Neurometer（R）测量A β和A δ神经纤维的电流感知阈值的显著改善。此外，C型神经纤维达到了相同的电流感知阈值比小鼠皮肤，在所有重建的皮肤富集或不与层粘连蛋白。我们的结论是，使用富含层粘连蛋白的组织工程化自体皮肤移植物有可能有效地优化皮肤感觉神经再生体内。(c)2006爱思唯尔有限公司版权所有。
The use of autologous reconstructed skin appears to be a promising treatment for the permanent coverage of deep and extensive burns. However, the capability of reconstructed skin transplanted on wounds to promote recovery of sensory perception is a major concern. Our aim was to assess the effect of laminin on cutaneous nerve regeneration. We prepared collagen-chitosan sponges enriched with 0, 1, 10 or 50 mu g of laminin/sponge to produce tissue-engineered reconstructed skins by culture of human fibroblasts and keratinocytes, then grafted on the back of athymic mice for 120 days. Immunohistochemical studies demonstrated that there were 7 times more neurofilament 150 kD-positive nerve fibers migrating in the graft in the samples enriched with 10 mu g laminin/sponge, compared to reconstructed skin without laminin, 120 days after graft. A significant improvement in the current perception threshold of the A beta and A delta nerve fibers was measured using a Neurometer (R) in all grafts enriched with laminin. In addition, the type C nerve fibers reached an identical current perception threshold than mouse skin, in all reconstructed skins enriched or not with laminin. We conclude that the use of a tissue-engineered autologous skin graft enriched with laminin has the potential to efficiently optimize cutaneous sensory nerve regeneration in vivo. (c) 2006 Elsevier Ltd. All rights reserved.