The microbiota in the intestinal and respiratory tracts of naked mole-rats revealed by high-throughput sequencing.
The microbiota in the intestinal and respiratory tracts of naked mole-rats revealed by high-throughput sequencing.
复制标题
高通量测序揭示裸鼹鼠肠道和呼吸道微生物群
DOI：
10.1186/s12866-018-1226-4
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发表时间：
2018-08-22
期刊：
影响因子：
4.2
通讯作者：
Cui S
中科院分区：
文献类型：
--
作者：
Cong W;Xing J;Feng Y;Wang J;Fu R;Yue B;He Z;Lin L;Yang W;Cheng J;Sun W;Cui S
裸鼹鼠（NMR，Heterocephalusglaber）是一种新型的实验动物，具有长寿、抗癌、耐缺氧、对疼痛不敏感等独特的生物学特性。预期野生NMR的微生物群与人工环境中NMR的微生物群之间存在差异。总体而言，环境对NMR微生物群变化的影响仍然未知。在试图了解的微生物群组成的NMRs在圈养，变异的肠道和呼吸道的微生物的两组NMRs进行了评估，在两种conditions.ResultsThe结果获得的高通量测序揭示了显着差异，在门，纲，目，科和属的水平之间的微生物群的两组NMRs检查（第一组在常规环境中，第二组在屏障环境中）。对于气管，第一组和第二组动物分别鉴定出24门和533属以及26门和733属。关于盲肠，在第一组和第二组动物的微生物群中鉴定出23门和385属以及25门和110属。有没有明显的差异，女性和男性或年轻和成年animals.ConclusionsOur结果表明，肠道和呼吸道NMR菌群的变化在圈养期间，这可能与过渡到繁殖环境。NMR菌群的这种变化可能会对原有特性产生影响，这可能是进一步研究的方向。
BackgroundThe naked mole-rat (NMR,Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMRs in an artificial environment. Overall, the effect of environment on changes in the NMR microbiota remains unknown. In an attempt to understand the microbiota composition of NMRs in captivity, variability in the microbiota of the intestinal and respiratory tracts of two groups of NMRs was assessed under two conditions.ResultsThe results obtained by high-throughput sequencing revealed significant differences at the phylum, class, order, family and genus levels in the microbiota between the two groups of NMRs examined (first group in conventional environment, second group in barrier environment). For the trachea, 24 phyla and 533 genera and 26 phyla and 733 genera were identified for the first and second groups of animals. Regarding the cecum, 23 phyla and 385 genera and 25 phyla and 110 genera were identified in the microbiota of first and second groups of animals. There were no obvious differences between females and males or young and adult animals.ConclusionsOur results suggest that the intestinal and respiratory tract NMR microbiota changed during captivity, which may be related to the transition to the breeding environment. Such changes in the microbiota of NMRs may have an effect on the original characteristics, which may be the direction of further research studies.