Arbuscular mycorrhizal fungal community of wheat under long-term mineral and organic amendments in semi-arid Mediterranean Turkey
Arbuscular mycorrhizal fungal community of wheat under long-term mineral and organic amendments in semi-arid Mediterranean Turkey
复制标题
半干旱地中海土耳其长期矿物和有机改良下小麦丛枝菌根真菌群落
DOI：
10.1080/15324982.2016.1177749
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发表时间：
2016
影响因子：
1.4
通讯作者：
Nonaka M
中科院分区：
文献类型：
--
作者：
Buto T;Suzuki K;Kaidzu T;Narisawa T;Turgay OC;Ortas I;Harada N;Nonaka M
关于丛枝菌根(AM)真菌与土耳其半干旱地区作物之间的联系，目前可获得的信息很少。因此，我们利用分子生态学技术，研究了不同管理措施(不施肥、不施化肥、不施农家肥、不加植物堆肥改良剂)对小麦根际AM真菌群落的影响。试验是在1996年在地中海土耳其南部建立的一块田地上进行的，由于不利的气候影响和土壤特性，那里的土壤生产力很低。我们测定了201个AM真菌核糖体大亚基基因的部分序列。与化肥和农家肥处理相比，不施肥处理和植物堆肥处理的AM真菌丰富度较高。在所有处理中都发现了与食根菌相关的无性系，占AM真菌无性系总数的37%，而在化肥条件下，根型无性系占主导地位。基于可操作分类单元频率的冗余度分析表明，AM真菌群落可分为3类，即对照处理、化肥处理和有机处理(农家肥和植物堆肥处理)。尽管不同的有机改良剂支持的AM真菌群落相对相似，但植物堆肥诱导的AM真菌丰富度高于农家肥。
A minimal amount of information is currently available concerning arbuscular mycorrhizal (AM) fungal associations with crops in semi-arid zones on Leptosols in Turkey. Therefore, using molecular ecological techniques, we studied the effects of different management practices (without fertilization, chemical fertilization, farmyard manure, and plant compost amendments) on AM fungal communities associated with wheat roots. Experiments were conducted in a field established in 1996 in southern Mediterranean Turkey where soil productivity is low owing to unfavorable climatic effects and soil characteristics. We determined 201 partial sequences of AM fungal nuclear ribosomal large subunit genes. The higher AM fungal richness was found in the control treatment without fertilization and plant compost treatments compared with the chemical fertilization and farmyard manure treatments. Clones related toRhizophaguswere found in all treatments and accounted for 37% of the total AM fungal clones, whereas those ofFunneliformiswere dominant under chemical fertilization. Redundancy analysis based on the frequency of operational taxonomic units revealed that AM fungal communities were divided into three groups, namely, the control treatment, the chemical fertilization treatment, and the organic treatments (farmyard manure and plant compost treatments). Although different organic amendments supported relatively similar AM fungal communities, plant compost induced higher AM fungal richness than farmyard manure fertilization.